hCG对人子宫内膜膜联蛋白IV和VEGF调控的体外研究。

IF 3.4 Q2 BIOCHEMICAL RESEARCH METHODS
Biochemistry Research International Pub Date : 2020-10-23 eCollection Date: 2020-01-01 DOI:10.1155/2020/8892930
Shaoyuan Xu, Jie Li, Xiaoyan Chen, Beiyu Liu
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引用次数: 5

摘要

目的:植入过程中血管内皮生长因子(VEGF)和膜联蛋白IV的变化是否通过LH/hCG-R调控尚需进一步研究。探讨hCG对人子宫内膜细胞膜联蛋白IV和VEGF表达的影响机制。方法:从人子宫内膜标本中分离鉴定子宫内膜细胞。分析腺细胞和上皮细胞的比例。采用qRT-PCR (mRNA)、western blot(蛋白)、免疫组化(蛋白)分析Annexin IV和VEGF。免疫组化法确定蛋白定位。细胞分别用hCG、hCG/PD98059(一种MAPK抑制剂)或不处理(对照组)培养。结果:接种和添加hCG时腺上皮细胞与间质细胞的比例分别为25.8±0.2%和27.8±0.04% (P > 0.05)。子宫内膜细胞胞浆中可见LH/hCG-R、膜联蛋白IV、VEGF。hCG治疗2、6、12、24 h后,VEGF mRNA与1 h相比分别升高1.25倍、3.19倍、4.21倍、4.86倍,annexin IV分别升高2.23倍、3.37倍、5.14倍、5.02倍。与对照组相比,hCG组和hCG/PD98059组annexin IV mRNA和蛋白含量升高(mRNA/蛋白含量分别为1.99倍/1.80倍和2.33倍/1.93倍,P < 0.05)。与对照组比较,hCG组VEGF mRNA和VEGF蛋白升高(mRNA/蛋白比值:2.30倍/1.86倍),而hCG/PD98059组VEGF mRNA和VEGF蛋白无升高。结论:hCG可上调膜联蛋白IV和VEGF的mRNA和蛋白表达。PD98059不能抑制hCG对膜联蛋白IV的上调,但能抑制hCG对VEGF的上调。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

In Vitro Study on the Regulation of Annexin IV and VEGF by hCG in the Human Endometrium.

In Vitro Study on the Regulation of Annexin IV and VEGF by hCG in the Human Endometrium.

In Vitro Study on the Regulation of Annexin IV and VEGF by hCG in the Human Endometrium.

In Vitro Study on the Regulation of Annexin IV and VEGF by hCG in the Human Endometrium.

Objective: Whether changes in vascular endothelial growth factor (VEGF) and annexin IV during implantation are regulated through the LH/hCG-R needs further research. To investigate the mechanism of hCG on the expression of annexin IV and VEGF in human endometrial cells.

Methods: Endometrial cells were isolated and identified from human specimens. The proportion of glandular and epithelial cells was analyzed. Annexin IV and VEGF were analyzed by qRT-PCR (mRNA), western blot (proteins), and immunohistochemistry (proteins). Protein location was identified by immunohistochemistry. The cells were cultured with hCG, hCG/PD98059 (a MAPK inhibitor), or no treatment (control).

Results: The proportions between the glandular epithelial cells and stromal cells at inoculation and when adding hCG were 25.8 ± 0.2% and 27.8 ± 0.04%, respectively (P > 0.05). LH/hCG-R, annexin IV, and VEGF were found in the cytoplasm of endometrial cells. After 2, 6, 12, and 24 h of hCG treatment, compared with 1 h, VEGF mRNA was increased by 1.25-fold, 3.19-fold, 4.21-fold, and 4.86-fold and annexin IV by 2.23-fold, 3.37-fold, 5.14-fold, and 5.02-fold. Compared with the control group, annexin IV mRNA and protein were increased in the hCG and hCG/PD98059 groups (mRNA/protein: 1.99-fold/1.80-fold and 2.33-fold/1.93-fold, P < 0.05). Compared with the control group, VEGF mRNA and protein were increased in the hCG group (mRNA/protein: 2.30-fold/1.86-fold), but not in the hCG/PD98059 group.

Conclusion: hCG could upregulate the mRNA and protein expression of annexin IV and VEGF. The upregulation of annexin IV by hCG could not be inhibited by PD98059, but the upregulation of VEGF by hCG could.

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来源期刊
Biochemistry Research International
Biochemistry Research International BIOCHEMICAL RESEARCH METHODS-
CiteScore
6.30
自引率
0.00%
发文量
27
审稿时长
14 weeks
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