果蝇的单细胞 RNA 测序:技术与应用。

Q1 Biochemistry, Genetics and Molecular Biology
Hongjie Li
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引用次数: 0

摘要

单细胞 RNA 测序(scRNA-seq)已成为在单细胞水平研究细胞状态和功能的有力工具。它极大地改变了神经生物学、免疫学和发育生物学等许多生命科学研究领域的转录组研究。近十年来,随着实验平台和生物信息学方法的快速发展,scRNA-seq 已成为许多生物医学实验室经济上可行、实验上实用的方法。果蝇是研究组织发育、成体细胞功能、疾病和衰老的细胞和分子机制的极佳模式生物。最近,scRNA-seq 方法在果蝇组织中的应用带来了许多令人兴奋的发现。在这篇综述中,我将总结果蝇最近的 scRNA-seq 研究,重点是技术方法和生物学应用。我还将讨论利用果蝇 scRNA-seq 进行新发现的当前挑战和未来机遇。本文归类于技术 > 转录组分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Single-cell RNA sequencing in Drosophila: Technologies and applications.

Single-cell RNA sequencing in Drosophila: Technologies and applications.

Single-cell RNA sequencing in Drosophila: Technologies and applications.

Single-cell RNA sequencing in Drosophila: Technologies and applications.

Single-cell RNA sequencing (scRNA-seq) has emerged as a powerful tool for investigating cell states and functions at the single-cell level. It has greatly revolutionized transcriptomic studies in many life science research fields, such as neurobiology, immunology, and developmental biology. With the fast development of both experimental platforms and bioinformatics approaches over the past decade, scRNA-seq is becoming economically feasible and experimentally practical for many biomedical laboratories. Drosophila has served as an excellent model organism for dissecting cellular and molecular mechanisms that underlie tissue development, adult cell function, disease, and aging. The recent application of scRNA-seq methods to Drosophila tissues has led to a number of exciting discoveries. In this review, I will provide a summary of recent scRNA-seq studies in Drosophila, focusing on technical approaches and biological applications. I will also discuss current challenges and future opportunities of making new discoveries using scRNA-seq in Drosophila. This article is categorized under:   Technologies > Analysis of the Transcriptome.

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来源期刊
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期刊介绍: Developmental biology is concerned with the fundamental question of how a single cell, the fertilized egg, ultimately produces a complex, fully patterned adult organism. This problem is studied on many different biological levels, from the molecular to the organismal. Developed in association with the Society for Developmental Biology, WIREs Developmental Biology will provide a unique interdisciplinary forum dedicated to fostering excellence in research and education and communicating key advances in this important field. The collaborative and integrative ethos of the WIREs model will facilitate connections to related disciplines such as genetics, systems biology, bioengineering, and psychology. The topical coverage of WIREs Developmental Biology includes: Establishment of Spatial and Temporal Patterns; Gene Expression and Transcriptional Hierarchies; Signaling Pathways; Early Embryonic Development; Invertebrate Organogenesis; Vertebrate Organogenesis; Nervous System Development; Birth Defects; Adult Stem Cells, Tissue Renewal and Regeneration; Cell Types and Issues Specific to Plants; Comparative Development and Evolution; and Technologies.
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