一种改良的双传代法评价胁迫真菌孢子群体的双模式损伤。

IF 0.9 4区农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biocontrol science Pub Date : 2020-01-01 DOI:10.4265/bio.25.131

Shigetoshi Horikiri, Masakazu Furuta, Tetsuaki Tsuchido

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引用次数: 4

摘要

为了评价受胁迫真菌孢子群体的伤害，提出了一种由传统的平板计数法和生长延迟分析法组成的双重传代法。在该方法中，假设表观对数生长动力学，并对先前的动力学模型进行了改进，使其能够在两种不同的模式下估计受伤亚群，分别包含早期发生的生长无关和晚期发生的生长依赖损伤，分别称为λ和μ损伤。基于此建立的动力学理论，将该方法应用于枝孢子的热处理分生孢子，并对两种模式损伤进行了评价。

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A Modified Double Subculture Method for the Two-Mode Injuries Evaluation in a Stressed Fungal Spore Population.

In order to evaluate injury of a stressed fungal spore population, a modification of formerly presented double subculture method, which consists of both the conventional plate count method and the growth delay analysis method, was proposed. In this method, an apparent logarithmic growth kinetics was assumed and the previous kinetic model was improved to be able to estimate injured subpopulations in two different modes containing early occurring growth-independent and late occurring growth-dependent injuries, called the λ and μ injuries, respectively. Based on the kinetic theory developed here, this novel method was applied to heat-treated conidia of Cladosporium cladosporioides and these two mode injuries were evaluated.

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来源期刊

Biocontrol science BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

2.60

自引率

8.30%

发文量

审稿时长

>12 weeks

期刊介绍： The Biocontrol Science provides a medium for the publication of original articles, concise notes, and review articles on all aspects of science and technology of biocontrol.