DAB光氧化和锇黑DRAQ5标记细长聚球藻PCC 7942 DNA的透射电镜观察

IF 1.8 4区 工程技术
Microscopy Pub Date : 2021-06-01 DOI:10.1093/jmicro/dfaa058
Ilika Ghosh;Kimie Atsuzawa;Aoi Arai;Ryuzo Ohmukai;Yasuko Kaneko
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引用次数: 1

摘要

为了观察在细胞分裂前的规则光/暗周期中的特定时间出现的细长聚球藻PCC 7942的压实DNA的精细结构,应用了具有一些修饰的ChromEM。用DRAQ5染色DNA后,将细胞固定,并在3,3-二氨基联苯胺存在下用红色激光照射,随后用OsO4固定。建立了一个用He–Ne激光(633nm)有效照射水溶液中细菌细胞的系统。通过透射电子显微镜观察压实的DNA,在超薄切片中通过锇黑进行电子致密染色。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
TEM observation of compacted DNA of Synechococcus elongatus PCC 7942 using DRAQ5 labeling with DAB photooxidation and osmium black
To visualize the fine structure of compacted DNA of Synechococcus elongatus PCC 7942, which appears at a specific time in the regular light/dark cycle prior to cell division, ChromEM with some modifications was applied. After staining DNA with DRAQ5, the cells were fixed and irradiated by red laser in the presence of 3,3ʹ-diaminobenzidine and subsequently fixed with OsO 4 . A system with He–Ne laser (633 nm) was set up for efficient irradiation of the bacterial cells in aqueous solution. The compacted DNA was visualized by transmission electron microscopy, in ultrathin sections as electron dense staining by osmium black.
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来源期刊
Microscopy
Microscopy 工程技术-显微镜技术
自引率
11.10%
发文量
0
审稿时长
>12 weeks
期刊介绍: Microscopy, previously Journal of Electron Microscopy, promotes research combined with any type of microscopy techniques, applied in life and material sciences. Microscopy is the official journal of the Japanese Society of Microscopy.
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