荆芥水酒精提取物对小鼠的催眠作用。

Q1 Agricultural and Biological Sciences
Hassan Rakhshandeh, Roghayeh Rashidi, Mohammad Mahdi Vahedi, Mohammad Bagher Khorrami, Hassan Abbassian, Fatemeh Forouzanfar
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引用次数: 3

摘要

背景:睡眠障碍是最常见的精神和医学疾病之一。草药在治疗睡眠障碍方面似乎是有效的,这已经被许多出版物和专利所重视。目的:研究刺毛椒水酒精提取物(HAE)对小鼠的催眠作用。方法:采用三种剂量的HAE(30、60、120 mg/kg),分别给予正己烷部分(NHF)、水部分(WF)、乙酸乙酯部分(EAF),并与阳性对照地西泮(3mg /kg体重)和阴性对照生理盐水进行比较。30min后给予戊巴比妥(30mg /kg体重i.p.)。此外,在1929个细胞中使用MTT法检测了HAE的LD50并评估了HAE的细胞毒性。此外,对于运动协调能力,在给药30分钟后,进行旋转试验。结果:HAE及各组分均能显著延长戊巴比妥诱导的睡眠时间,与地西泮诱导的睡眠时间相当。LD50值为2.4 g/kg。提取物对L929成纤维细胞无细胞毒作用。HAE不影响动物在旋转棒试验中的表现。结论:本研究提示棘草水醇提取物具有催眠作用,有待进一步的科学研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hypnotic Activity of Capparis spinosa Hydro-alcoholic Extract in Mice.

Background: Sleep disorders are among the most common psychiatric and medical conditions. Herbal medicine appears to be effective in the treatment of sleep disorders which have been valued by many of publications and patents.

Objective: The present study aimed at investigating the hypnotic activity of the hydro-alcoholic extract of Capparis spinosa (HAE) in mice.

Methods: Three doses of HAE (30, 60 and 120 mg/kg) and three fractions of it, namely n-hexane fraction (NHF), water fraction (WF), and ethyl acetate fraction (EAF), were given in comparison with diazepam (3 mg/kg body weight i.p.) as a positive control and saline as a negative control. After 30 min, pentobarbital (30 mg/kg body weight i.p.) was administered. In addition, LD50 of HAE was examined and the cytotoxicity of HAE was assessed in l929 cells using the MTT assay. Moreover, for motorcoordination ability, 30 mins after administration of HAE, the rotarod test was performed.

Results: The results exhibited that the HAE and all the fractions significantly augmented pentobarbital induced sleeping time, which was comparable to that of induced by diazepam. The LD50 value was 2.4 g/kg. The extract did not induce any cytotoxic effects in L929 fibroblast cells. HAE did not affect the animals' performance on the rotarod test.

Conclusion: Our finding suggests that the hydro-alcoholic extract of C. spinosa possesses a hypnotic potential that may require further scientific investigations.

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来源期刊
Recent patents on food, nutrition & agriculture
Recent patents on food, nutrition & agriculture Agricultural and Biological Sciences-Agronomy and Crop Science
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