A Riahi, M Hosseinpour-Feizi, A Rajabi, M Akbarzadeh, V Montazeri, R Safaralizadeh
{"title":"与邻近非肿瘤组织相比，长链非编码RNA MCM3AP-AS1在乳腺癌组织中的过表达","authors":"A Riahi, M Hosseinpour-Feizi, A Rajabi, M Akbarzadeh, V Montazeri, R Safaralizadeh","doi":"10.1080/09674845.2020.1798058","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Altered expression of several long non-coding RNAs (lncRNAs) has been described in numerous malignancies, including breast cancer, and some may have a role in carcinogenesis. We hypothesised differences in the expression of lncRNA MCM3AP-AS1 in breast cancer tissues compared to nearby healthy tissues and potential links with clinical features.</p><p><strong>Methods: </strong>We tested our hypothesis in 102 pairs of breast cancer tumours and adjacent non-tumour tissues from female patients. After RNA extraction, cDNA synthesis was performed for all specimens. The differential gene expression was assessed using Quantitative Real-Time PCR Technique.</p><p><strong>Results: </strong>There was a significant overexpression of the lncRNAs in tumour tissues as compared with their adjacent non-tumour tissues (<i>P</i> < 0.001). Expression was significantly linked with the tumour oestrogen receptor expression (<i>P</i> = 0.023) and tumour progesterone receptor expression (<i>P</i> < 0.001). ROC analysis showed an AUC of 0.67 (95% CI 0.60-0.75) (<i>P</i> < 0.001) with sensitivity and specificity of 58% and 76%, respectively.</p><p><strong>Conclusion: </strong>The lncRNA MCM3AP-AS1 may be a novel breast cancer lncRNA with high expression levels in breast cancer patients' tissue. Further investigations are needed to confirm its uses as a potential molecular marker and therapeutic target.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 2","pages":"53-57"},"PeriodicalIF":2.7000,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1798058","citationCount":"13","resultStr":"{\"title\":\"Overexpression of long non-coding RNA MCM3AP-AS1 in breast cancer tissues compared to adjacent non-tumour tissues.\",\"authors\":\"A Riahi, M Hosseinpour-Feizi, A Rajabi, M Akbarzadeh, V Montazeri, R Safaralizadeh\",\"doi\":\"10.1080/09674845.2020.1798058\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Altered expression of several long non-coding RNAs (lncRNAs) has been described in numerous malignancies, including breast cancer, and some may have a role in carcinogenesis. We hypothesised differences in the expression of lncRNA MCM3AP-AS1 in breast cancer tissues compared to nearby healthy tissues and potential links with clinical features.</p><p><strong>Methods: </strong>We tested our hypothesis in 102 pairs of breast cancer tumours and adjacent non-tumour tissues from female patients. After RNA extraction, cDNA synthesis was performed for all specimens. The differential gene expression was assessed using Quantitative Real-Time PCR Technique.</p><p><strong>Results: </strong>There was a significant overexpression of the lncRNAs in tumour tissues as compared with their adjacent non-tumour tissues (<i>P</i> < 0.001). Expression was significantly linked with the tumour oestrogen receptor expression (<i>P</i> = 0.023) and tumour progesterone receptor expression (<i>P</i> < 0.001). ROC analysis showed an AUC of 0.67 (95% CI 0.60-0.75) (<i>P</i> < 0.001) with sensitivity and specificity of 58% and 76%, respectively.</p><p><strong>Conclusion: </strong>The lncRNA MCM3AP-AS1 may be a novel breast cancer lncRNA with high expression levels in breast cancer patients' tissue. Further investigations are needed to confirm its uses as a potential molecular marker and therapeutic target.</p>\",\"PeriodicalId\":9236,\"journal\":{\"name\":\"British Journal of Biomedical Science\",\"volume\":\"78 2\",\"pages\":\"53-57\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2021-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1080/09674845.2020.1798058\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"British Journal of Biomedical Science\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/09674845.2020.1798058\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2020/9/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"British Journal of Biomedical Science","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/09674845.2020.1798058","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/9/7 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
Overexpression of long non-coding RNA MCM3AP-AS1 in breast cancer tissues compared to adjacent non-tumour tissues.
Background: Altered expression of several long non-coding RNAs (lncRNAs) has been described in numerous malignancies, including breast cancer, and some may have a role in carcinogenesis. We hypothesised differences in the expression of lncRNA MCM3AP-AS1 in breast cancer tissues compared to nearby healthy tissues and potential links with clinical features.
Methods: We tested our hypothesis in 102 pairs of breast cancer tumours and adjacent non-tumour tissues from female patients. After RNA extraction, cDNA synthesis was performed for all specimens. The differential gene expression was assessed using Quantitative Real-Time PCR Technique.
Results: There was a significant overexpression of the lncRNAs in tumour tissues as compared with their adjacent non-tumour tissues (P < 0.001). Expression was significantly linked with the tumour oestrogen receptor expression (P = 0.023) and tumour progesterone receptor expression (P < 0.001). ROC analysis showed an AUC of 0.67 (95% CI 0.60-0.75) (P < 0.001) with sensitivity and specificity of 58% and 76%, respectively.
Conclusion: The lncRNA MCM3AP-AS1 may be a novel breast cancer lncRNA with high expression levels in breast cancer patients' tissue. Further investigations are needed to confirm its uses as a potential molecular marker and therapeutic target.
期刊介绍：
The British Journal of Biomedical Science is committed to publishing high quality original research that represents a clear advance in the practice of biomedical science, and reviews that summarise recent advances in the field of biomedical science. The overall aim of the Journal is to provide a platform for the dissemination of new and innovative information on the diagnosis and management of disease that is valuable to the practicing laboratory scientist.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
