评估Hsp90基因在推断纤毛虫亚纲(原生动物,纤毛虫)进化关系中的效用。

IF 3.4 Q1 Agricultural and Biological Sciences
Qi Zhang, Jiahui Xu, Alan Warren, Ran Yang, Zhuo Shen, Zhenzhen Yi
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引用次数: 0

摘要

背景:尽管系统基因组学分析越来越多地用于揭示纤毛虫之间的进化关系,但相对较少的核蛋白编码基因标记已被测试用于推断该类群的系统发生。在这项研究中,我们研究了热休克蛋白90基因(Hsp90)作为推断低营养纤毛虫之间系统发育关系的标记的效用。结果:共获得10个欠营养种的87条Hsp90新基因序列。其中85个是不同的序列。基于这些数据的系统发育分析表明:(1)Hsp90基因氨基酸树在恢复类级的系统发育关系方面与小亚基rDNA树相当,但在推断属级的进化关系方面缺乏足够的系统发育信号;(2) Hsp90基因同源物是最近发现的,因此不太可能对恢复低营养枝构成重大问题;(3)由于某些亚富目和科的单系性没有得到各种基因标记的支持,需要重新定义;(4)散柄虫目为副虫目,但“核心”尿柄虫目的单系性得到支持;(5)木刺虫亚科和尿瘤属似乎都是非单系的,但尿瘤的单系性并没有被AU测试所拒绝。结论:我们的研究结果首次证明了Hsp90基因与SSU rDNA在恢复类级系统发育关系方面具有可比性,其相似物不太可能对恢复低营养进化支构成重大问题。本研究显示了精心选择基因标记对纤毛虫系统基因组分析的价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Assessing the utility of Hsp90 gene for inferring evolutionary relationships within the ciliate subclass Hypotricha (Protista, Ciliophora).

Assessing the utility of Hsp90 gene for inferring evolutionary relationships within the ciliate subclass Hypotricha (Protista, Ciliophora).

Assessing the utility of Hsp90 gene for inferring evolutionary relationships within the ciliate subclass Hypotricha (Protista, Ciliophora).

Assessing the utility of Hsp90 gene for inferring evolutionary relationships within the ciliate subclass Hypotricha (Protista, Ciliophora).

Background: Although phylogenomic analyses are increasingly used to reveal evolutionary relationships among ciliates, relatively few nuclear protein-coding gene markers have been tested for their suitability as candidates for inferring phylogenies within this group. In this study, we investigate the utility of the heat-shock protein 90 gene (Hsp90) as a marker for inferring phylogenetic relationships among hypotrich ciliates.

Results: A total of 87 novel Hsp90 gene sequences of 10 hypotrich species were generated. Of these, 85 were distinct sequences. Phylogenetic analyses based on these data showed that: (1) the Hsp90 gene amino acid trees are comparable to the small subunit rDNA tree for recovering phylogenetic relationships at the rank of class, but lack sufficient phylogenetic signal for inferring evolutionary relationships at the genus level; (2) Hsp90 gene paralogs are recent and therefore unlikely to pose a significant problem for recovering hypotrich clades; (3) definitions of some hypotrich orders and families need to be revised as their monophylies are not supported by various gene markers; (4) The order Sporadotrichida is paraphyletic, but the monophyly of the "core" Urostylida is supported; (5) both the subfamily Oxytrichinae and the genus Urosoma seem to be non-monophyletic, but monophyly of Urosoma is not rejected by AU tests.

Conclusions: Our results for the first time demonstrate that the Hsp90 gene is comparable to SSU rDNA for recovering phylogenetic relationships at the rank of class, and its paralogs are unlikely to pose a significant problem for recovering hypotrich clades. This study shows the value of careful gene marker selection for phylogenomic analyses of ciliates.

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来源期刊
BMC Evolutionary Biology
BMC Evolutionary Biology 生物-进化生物学
CiteScore
5.80
自引率
0.00%
发文量
0
审稿时长
6 months
期刊介绍: BMC Evolutionary Biology is an open access, peer-reviewed journal that considers articles on all aspects of molecular and non-molecular evolution of all organisms, as well as phylogenetics and palaeontology.
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