{"title":"意大利腹泻患儿缺乏HPyV12 DNA实时PCR检测。","authors":"Valentina Daprà, Ilaria Galliano, Marco Rassu, Cristina Calvi, Paola Montanari, Chiara Merlino, Massimiliano Bergallo","doi":"10.23736/S2724-5276.20.05738-2","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>HPyV12 was found in organs of the digestive tract, in particular the liver but also in colon, rectum and feces. Until now, the prevalence of HPyV12 is not well characterized.</p><p><strong>Methods: </strong>In this study, we investigate the presence of this novel polyomavirus DNA in stool specimens collected from under-five-year-old children with gastroenteritis compared to healthy infants. A total of 190 fecal specimens previously screened for rotavirus (RV) and adenovirus (ADV) and 80 fecal samples from healthy infants, were tested for HPyV12 DNA using a home-made real time PCR. All fecal specimens were tested for the presence of HPyV12 with specific primers and probes.</p><p><strong>Results: </strong>None of 190 (0%) episodes of acute gastroenteritis was associated with HPyV12. We did not detect HPyV12 DNA in any of 80 control subjects, as well.</p><p><strong>Conclusions: </strong>Our study represents a pilot study aiming to clarify the current epidemiological pattern in pediatric Italian patients regarding the novel and rare HPyV12. Based on our negative data and the recent observations reported in literature, doubts remain on human tropism of the HPyV12 and epidemiology: these issues need further investigations.</p>","PeriodicalId":18533,"journal":{"name":"Minerva pediatrica","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Lack of detection of HPyV12 DNA using real-time PCR in Italian infants with diarrhea.\",\"authors\":\"Valentina Daprà, Ilaria Galliano, Marco Rassu, Cristina Calvi, Paola Montanari, Chiara Merlino, Massimiliano Bergallo\",\"doi\":\"10.23736/S2724-5276.20.05738-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>HPyV12 was found in organs of the digestive tract, in particular the liver but also in colon, rectum and feces. Until now, the prevalence of HPyV12 is not well characterized.</p><p><strong>Methods: </strong>In this study, we investigate the presence of this novel polyomavirus DNA in stool specimens collected from under-five-year-old children with gastroenteritis compared to healthy infants. A total of 190 fecal specimens previously screened for rotavirus (RV) and adenovirus (ADV) and 80 fecal samples from healthy infants, were tested for HPyV12 DNA using a home-made real time PCR. All fecal specimens were tested for the presence of HPyV12 with specific primers and probes.</p><p><strong>Results: </strong>None of 190 (0%) episodes of acute gastroenteritis was associated with HPyV12. We did not detect HPyV12 DNA in any of 80 control subjects, as well.</p><p><strong>Conclusions: </strong>Our study represents a pilot study aiming to clarify the current epidemiological pattern in pediatric Italian patients regarding the novel and rare HPyV12. Based on our negative data and the recent observations reported in literature, doubts remain on human tropism of the HPyV12 and epidemiology: these issues need further investigations.</p>\",\"PeriodicalId\":18533,\"journal\":{\"name\":\"Minerva pediatrica\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Minerva pediatrica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.23736/S2724-5276.20.05738-2\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2020/6/5 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Minerva pediatrica","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.23736/S2724-5276.20.05738-2","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/6/5 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
摘要
背景:HPyV12主要存在于消化道器官，尤其是肝脏，但也存在于结肠、直肠和粪便中。到目前为止，HPyV12的流行还没有很好的特征。方法:在这项研究中，我们调查了这种新型多瘤病毒DNA在五岁以下肠胃炎儿童粪便标本中的存在，并与健康婴儿进行了比较。利用自制的实时聚合酶链反应(real - time PCR)对190份先前筛选过轮状病毒(RV)和腺病毒(ADV)的粪便样本和80份健康婴儿的粪便样本进行HPyV12 DNA检测。用特异性引物和探针检测所有粪便标本是否存在HPyV12。结果:190例(0%)急性胃肠炎发作与HPyV12无关。我们在80名对照受试者中也未检测到HPyV12 DNA。结论:我们的研究是一项初步研究，旨在阐明目前意大利儿科患者中关于新型和罕见HPyV12的流行病学模式。根据我们的阴性数据和最近文献报道的观察结果，对HPyV12的人类倾向和流行病学仍然存在疑问:这些问题需要进一步调查。
Lack of detection of HPyV12 DNA using real-time PCR in Italian infants with diarrhea.
Background: HPyV12 was found in organs of the digestive tract, in particular the liver but also in colon, rectum and feces. Until now, the prevalence of HPyV12 is not well characterized.
Methods: In this study, we investigate the presence of this novel polyomavirus DNA in stool specimens collected from under-five-year-old children with gastroenteritis compared to healthy infants. A total of 190 fecal specimens previously screened for rotavirus (RV) and adenovirus (ADV) and 80 fecal samples from healthy infants, were tested for HPyV12 DNA using a home-made real time PCR. All fecal specimens were tested for the presence of HPyV12 with specific primers and probes.
Results: None of 190 (0%) episodes of acute gastroenteritis was associated with HPyV12. We did not detect HPyV12 DNA in any of 80 control subjects, as well.
Conclusions: Our study represents a pilot study aiming to clarify the current epidemiological pattern in pediatric Italian patients regarding the novel and rare HPyV12. Based on our negative data and the recent observations reported in literature, doubts remain on human tropism of the HPyV12 and epidemiology: these issues need further investigations.
期刊介绍：
Minerva Pediatrica publishes scientific papers on pediatrics, neonatology, adolescent medicine, child and adolescent psychiatry and pediatric surgery. Manuscripts may be submitted in the form of editorials, original articles, review articles, special articles, letters to the Editor and guidelines. The journal aims to provide its readers with papers of the highest quality and impact through a process of careful peer review and editorial work.
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