费氏弧菌:实验室培养、储存和常见表型分析

Current Protocols in Microbiology Pub Date : 2020-06-04 DOI:10.1002/cpmc.103

David G. Christensen, Karen L. Visick

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引用次数: 13

摘要

费氏弧菌是一种与致病性弧菌相关的非致病性生物，可以很容易地在普通实验室设备中生长和储存。本文介绍了菲氏弧菌的常规生长、储存和表型评估方案，以及有用培养基的配方。具体来说，本文描述了该微生物在复杂和最小培养基中生长的程序和注意事项。它还描述了测定的生物膜形成，运动性和生物发光，三种常见的评估表型的费氏弧菌。©2020 Wiley期刊有限公司基本方案1:从冷冻储存库中生长的V. fischeri基本方案2:在丰富的，未定义的液体培养基中生长的V. fischeri备用方案1:在最小培养基中生长的V. fischeri基本方案3:在冷冻储存库中储存的V. fischeri基本方案4:在固体agon上的生物膜试验备用方案2:在晃动液体培养中进行生物膜试验备用方案3:在静态液体培养中进行生物膜试验基本方案5:活性试验基本方案6:发光分析

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Vibrio fischeri: Laboratory Cultivation, Storage, and Common Phenotypic Assays

Vibrio fischeri is a nonpathogenic organism related to pathogenic Vibrio species that can be readily grown and stored with common laboratory equipment. In this article, protocols for routine growth, storage, and phenotypic assessment of V. fischeri, as well as recipes for useful media, are included. Specifically, this article describes procedures and considerations for growth of this microbe in complex and minimal media. It also describes assays for biofilm formation, motility, and bioluminescence, three commonly assessed phenotypes of V. fischeri. © 2020 Wiley Periodicals LLC.

Basic Protocol 1: Growth of V. fischeri from frozen stocks

Basic Protocol 2: Growth of V. fischeri in rich, undefined liquid medium

Alternate Protocol 1: Growth of V. fischeri in minimal medium

Basic Protocol 3: Storage of V. fischeri in frozen stocks

Basic Protocol 4: Biofilm assay on solid agar

Alternate Protocol 2: Biofilm assay in shaking liquid culture

Alternate Protocol 3: Biofilm assay in static liquid culture

Basic Protocol 5: Motility assay

Basic Protocol 6: Luminescence assay

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来源期刊

Current Protocols in Microbiology Immunology and Microbiology-Parasitology

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期刊介绍： Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.