细胞周期分析技术的调色板。

IF 3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Anna E Eastman, Shangqin Guo
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引用次数: 0

摘要

细胞分裂周期是细胞生长和繁殖的一代时期。细胞周期的进展需要高度调控,以保持基因组的保真度,同时增加细胞数量。在多细胞生物体中,细胞周期还必须与发育和组织稳态过程中的细胞命运分化相协调。细胞周期动态变化在一些病理生理过程中也起着核心作用。因此,人们一直在努力确定执行细胞周期及其调控的生化机制,并实施更灵敏、更准确的细胞周期测量。在此,我们回顾了现有的细胞周期分析技术,重新审视了传统的基于群体的测量方法背后的假设,并讨论了在单细胞时代更好地处理细胞周期异质性的新工具。我们权衡了细胞周期时间测量方法的优缺点和利弊得失。最后,我们讨论了在活体动物中以单细胞分辨率捕捉细胞周期速度的新兴技术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The palette of techniques for cell cycle analysis.

The cell division cycle is the generational period of cellular growth and propagation. Cell cycle progression needs to be highly regulated to preserve genomic fidelity while increasing cell number. In multicellular organisms, the cell cycle must also coordinate with cell fate specification during development and tissue homeostasis. Altered cell cycle dynamics play a central role also in a number of pathophysiological processes. Thus, extensive effort has been made to define the biochemical machineries that execute the cell cycle and their regulation, as well as implementing more sensitive and accurate cell cycle measurements. Here, we review the available techniques for cell cycle analysis, revisiting the assumptions behind conventional population-based measurements and discussing new tools to better address cell cycle heterogeneity in the single-cell era. We weigh the strengths, weaknesses, and trade-offs of methods designed to measure temporal aspects of the cell cycle. Finally, we discuss emerging techniques for capturing cell cycle speed at single-cell resolution in live animals.

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来源期刊
FEBS Letters
FEBS Letters 生物-生化与分子生物学
CiteScore
6.60
自引率
2.90%
发文量
303
审稿时长
1 months
期刊介绍: FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.
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