{"title":"用含Caspofungin的快速比色微稀释法评价念珠菌的敏感性。","authors":"Nobuyoshi Noguchi, Akihiro Nakamura, Masaru Komatsu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>We carried out performance evaluation tests using clinical isolates in a modified yeast fungal drug susceptibility test kit ASTY plus caspofungin (CPFG) (Kyokuto Pharmaceutical Industry Co., Ltd., Tokyo, Japan). We used 51 strains of clinical isolates (<i>Candida albicans</i>, 30 strains; <i>C. dubliniensis</i>, 2 strains; <i>C. glabrata</i>, 4 strains; <i>C. tropicalis</i>, 4 strains; <i>C. guilliermondii</i>, 4 strains; <i>C. parapsilosis</i>, 4 strains; <i>C. krusei</i>, 3 strains). In this evaluation, minimum inhibitory concentration values of clinical isolates using the control CLSI method (Yeast-like fungi FP; Eiken Chemical, Tokyo, Japan), ASTY method, and another method (Yeast-like fungus DP; Eiken Chemical) were compared. The drugs with a concordance rate of 90% or more within ±1 tube were CPFG, amphotericin-B, 5-flucytosine, voriconazole, and those with a concordance rate of 80% or more were micafungin, fluconazole, and itraconazole. The above results clarified that the ASTY method correlated highly with the CLSI method.</p>","PeriodicalId":74740,"journal":{"name":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","volume":"29 2","pages":"59-64"},"PeriodicalIF":0.0000,"publicationDate":"2020-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Evaluation of <i>Candida</i> spp. Susceptibility Using Asty Colorimetric Microdilution Including Caspofungin].\",\"authors\":\"Nobuyoshi Noguchi, Akihiro Nakamura, Masaru Komatsu\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We carried out performance evaluation tests using clinical isolates in a modified yeast fungal drug susceptibility test kit ASTY plus caspofungin (CPFG) (Kyokuto Pharmaceutical Industry Co., Ltd., Tokyo, Japan). We used 51 strains of clinical isolates (<i>Candida albicans</i>, 30 strains; <i>C. dubliniensis</i>, 2 strains; <i>C. glabrata</i>, 4 strains; <i>C. tropicalis</i>, 4 strains; <i>C. guilliermondii</i>, 4 strains; <i>C. parapsilosis</i>, 4 strains; <i>C. krusei</i>, 3 strains). In this evaluation, minimum inhibitory concentration values of clinical isolates using the control CLSI method (Yeast-like fungi FP; Eiken Chemical, Tokyo, Japan), ASTY method, and another method (Yeast-like fungus DP; Eiken Chemical) were compared. The drugs with a concordance rate of 90% or more within ±1 tube were CPFG, amphotericin-B, 5-flucytosine, voriconazole, and those with a concordance rate of 80% or more were micafungin, fluconazole, and itraconazole. The above results clarified that the ASTY method correlated highly with the CLSI method.</p>\",\"PeriodicalId\":74740,\"journal\":{\"name\":\"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology\",\"volume\":\"29 2\",\"pages\":\"59-64\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-04-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rinsho Biseibutsu Jinsoku Shindan Kenkyukai shi = JARMAM : Journal of the Association for Rapid Method and Automation in Microbiology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
我们使用改良酵母真菌药敏试验试剂盒(ASTY + caspofungin, CPFG) (Kyokuto Pharmaceutical Industry Co.， Ltd, Tokyo, Japan)对临床分离株进行了性能评价试验。临床分离51株(白色念珠菌30株;dubliniensis, 2株;光斑草，4株;热带锥虫4株;吉列蒙地菌，4株;副枯枝孢菌4株;克鲁氏弧菌，3株)。在本评价中，使用对照CLSI方法分离的临床分离物的最小抑菌浓度值(酵母样真菌FP;Eiken Chemical, Tokyo, Japan)， ASTY法和另一种方法(酵母样真菌DP;艾肯化学公司)进行比较。±1管内符合率≥90%的药物为CPFG、两性霉素- b、5-氟胞嘧啶、伏立康唑，符合率≥80%的药物为米卡芬金、氟康唑、伊曲康唑。以上结果表明，ASTY方法与CLSI方法具有较高的相关性。
[Evaluation of Candida spp. Susceptibility Using Asty Colorimetric Microdilution Including Caspofungin].
We carried out performance evaluation tests using clinical isolates in a modified yeast fungal drug susceptibility test kit ASTY plus caspofungin (CPFG) (Kyokuto Pharmaceutical Industry Co., Ltd., Tokyo, Japan). We used 51 strains of clinical isolates (Candida albicans, 30 strains; C. dubliniensis, 2 strains; C. glabrata, 4 strains; C. tropicalis, 4 strains; C. guilliermondii, 4 strains; C. parapsilosis, 4 strains; C. krusei, 3 strains). In this evaluation, minimum inhibitory concentration values of clinical isolates using the control CLSI method (Yeast-like fungi FP; Eiken Chemical, Tokyo, Japan), ASTY method, and another method (Yeast-like fungus DP; Eiken Chemical) were compared. The drugs with a concordance rate of 90% or more within ±1 tube were CPFG, amphotericin-B, 5-flucytosine, voriconazole, and those with a concordance rate of 80% or more were micafungin, fluconazole, and itraconazole. The above results clarified that the ASTY method correlated highly with the CLSI method.
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