测量根系的水力传导性

Q1 Agricultural and Biological Sciences

Current protocols in plant biology Pub Date : 2020-04-20 DOI:10.1002/cppb.20110

Atara Gal, Elisha Hendel, Zvi Peleg, Nimrod Schwartz, Nir Sade

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引用次数: 2

摘要

根系水力传导性(RSHC)是一项重要的生理特性，描述了根系在根和茎木质部之间的水势梯度上传导水分的内在能力。RSHC通常被用作植物功能和对特定环境适应性的指标。本文描述了一种简单、快速、易于使用的方案，用于在水培溶液中生长的两种重要单子叶植物:C4模式植物绿尾草(Setaria viridis)和C3作物植物小麦(wheat)幼苗期RSHC的定量。该方案也可以很容易地修改，适用于几乎任何草种和环境处理，如盐度或激素处理。©2020 by John Wiley &基本方案:狗尾草静水根系导电性备用方案:测量软茎幼株的根系导电性

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Measuring the Hydraulic Conductivity of Grass Root Systems

Root-system hydraulic conductivity (RSHC) is an important physiological characteristic that describes the inherent ability of roots to conduct water across a water-potential gradient between the root and the stem xylem. RSHC is commonly used as an indicator of plant functioning and adaptability to a given environment. A simple, fast, and easy-to-use protocol is described for the quantification of RSHC at the seedling stage in two important monocot species grown in hydroponic solution: Setaria viridis, a C4 model plant, and wheat, a C3 crop plant. This protocol can also be easily modified for use with almost any grass species and environmental treatments, such as salinity or hormone treatments. © 2020 by John Wiley & Sons, Inc.

Basic Protocol: Setaria hydrostatic root-system hydraulic conductivity

Alternate Protocol: Measuring the root conductivity of young plants with soft stems

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来源期刊

Current protocols in plant biology Agricultural and Biological Sciences-Plant Science

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. Current Protocols in Plant Biology provides reproducible step-by-step instructions for protocols relevant to plant research. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols in Plant Biology to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Plant Biology is the comprehensive source for protocols in the multidisciplinary field of plant biology, providing an extensive range of protocols from basic to cutting edge. Coverage includes: Extraction and analysis of DNA, RNA, proteins Chromosome analysis Transcriptional analysis Protein expression Metabolites Plant enzymology Epigenetics Plant genetic transformation Mutagenesis Arabidopsis, Maize, Poplar, Rice, and Soybean, and more.