{"title":"约旦转基因玉米的检测。","authors":"Abeer Aburumman, Hussein Migdadi, Muhanad Akash, Ayed Al-Abdallat, Yaser Hassan Dewir, Muhammad Farooq","doi":"10.1080/21645698.2020.1747353","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to detect genetically modified maize (GMM) in seeds of eleven imported maize hybrids grown in Jordan. We used promoter <i>35 S</i> and <i>T-nos</i> terminator for general screening of transgenic materials. Conventional PCR detected the specific events for the screening of Bt 11, MON810, and Bt176 events. Seeds of eleven maize hybrids samples showed a positive response to the <i>35 S</i> promoter; nine out of eleven showed a positive response for <i>T-nos</i> terminator. Bt11 event was the most used in GMM seeds, where seven out of eleven samples showed positive results. Two out of eleven hybrids showed the presence of the Bt176 event; however, MON810 not detected in any of the tested hybrids. We studied the Bt11 event in imported GMM seeds in Jordan for the first time, reinforcing the need for a mandatory labeling system and a valid simple qualitative method in routine analysis of GMCs.</p>","PeriodicalId":54282,"journal":{"name":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","volume":"11 3","pages":"164-170"},"PeriodicalIF":4.5000,"publicationDate":"2020-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/21645698.2020.1747353","citationCount":"1","resultStr":"{\"title\":\"Detection of genetically modified maize in Jordan.\",\"authors\":\"Abeer Aburumman, Hussein Migdadi, Muhanad Akash, Ayed Al-Abdallat, Yaser Hassan Dewir, Muhammad Farooq\",\"doi\":\"10.1080/21645698.2020.1747353\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to detect genetically modified maize (GMM) in seeds of eleven imported maize hybrids grown in Jordan. We used promoter <i>35 S</i> and <i>T-nos</i> terminator for general screening of transgenic materials. Conventional PCR detected the specific events for the screening of Bt 11, MON810, and Bt176 events. Seeds of eleven maize hybrids samples showed a positive response to the <i>35 S</i> promoter; nine out of eleven showed a positive response for <i>T-nos</i> terminator. Bt11 event was the most used in GMM seeds, where seven out of eleven samples showed positive results. Two out of eleven hybrids showed the presence of the Bt176 event; however, MON810 not detected in any of the tested hybrids. We studied the Bt11 event in imported GMM seeds in Jordan for the first time, reinforcing the need for a mandatory labeling system and a valid simple qualitative method in routine analysis of GMCs.</p>\",\"PeriodicalId\":54282,\"journal\":{\"name\":\"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain\",\"volume\":\"11 3\",\"pages\":\"164-170\"},\"PeriodicalIF\":4.5000,\"publicationDate\":\"2020-07-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1080/21645698.2020.1747353\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1080/21645698.2020.1747353\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2020/4/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gm Crops & Food-Biotechnology in Agriculture and the Food Chain","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1080/21645698.2020.1747353","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2020/4/7 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Detection of genetically modified maize in Jordan.
This study aimed to detect genetically modified maize (GMM) in seeds of eleven imported maize hybrids grown in Jordan. We used promoter 35 S and T-nos terminator for general screening of transgenic materials. Conventional PCR detected the specific events for the screening of Bt 11, MON810, and Bt176 events. Seeds of eleven maize hybrids samples showed a positive response to the 35 S promoter; nine out of eleven showed a positive response for T-nos terminator. Bt11 event was the most used in GMM seeds, where seven out of eleven samples showed positive results. Two out of eleven hybrids showed the presence of the Bt176 event; however, MON810 not detected in any of the tested hybrids. We studied the Bt11 event in imported GMM seeds in Jordan for the first time, reinforcing the need for a mandatory labeling system and a valid simple qualitative method in routine analysis of GMCs.
期刊介绍：
GM Crops & Food - Biotechnology in Agriculture and the Food Chain aims to publish high quality research papers, reviews, and commentaries on a wide range of topics involving genetically modified (GM) crops in agriculture and genetically modified food. The journal provides a platform for research papers addressing fundamental questions in the development, testing, and application of transgenic crops. The journal further covers topics relating to socio-economic issues, commercialization, trade and societal issues. GM Crops & Food aims to provide an international forum on all issues related to GM crops, especially toward meaningful communication between scientists and policy-makers.
GM Crops & Food will publish relevant and high-impact original research with a special focus on novelty-driven studies with the potential for application. The journal also publishes authoritative review articles on current research and policy initiatives, and commentary on broad perspectives regarding genetically modified crops. The journal serves a wide readership including scientists, breeders, and policy-makers, as well as a wider community of readers (educators, policy makers, scholars, science writers and students) interested in agriculture, medicine, biotechnology, investment, and technology transfer.
Topics covered include, but are not limited to:
• Production and analysis of transgenic crops
• Gene insertion studies
• Gene silencing
• Factors affecting gene expression
• Post-translational analysis
• Molecular farming
• Field trial analysis
• Commercialization of modified crops
• Safety and regulatory affairs
BIOLOGICAL SCIENCE AND TECHNOLOGY
• Biofuels
• Data from field trials
• Development of transformation technology
• Elimination of pollutants (Bioremediation)
• Gene silencing mechanisms
• Genome Editing
• Herbicide resistance
• Molecular farming
• Pest resistance
• Plant reproduction (e.g., male sterility, hybrid breeding, apomixis)
• Plants with altered composition
• Tolerance to abiotic stress
• Transgenesis in agriculture
• Biofortification and nutrients improvement
• Genomic, proteomic and bioinformatics methods used for developing GM cops
ECONOMIC, POLITICAL AND SOCIAL ISSUES
• Commercialization
• Consumer attitudes
• International bodies
• National and local government policies
• Public perception, intellectual property, education, (bio)ethical issues
• Regulation, environmental impact and containment
• Socio-economic impact
• Food safety and security
• Risk assessments
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