荷斯坦奶牛过渡期维生素D受体、1 α -羟化酶和趋化因子RANTES基因表达模式与选定血清参数的关系

IF 1.3 Q2 VETERINARY SCIENCES
Veterinary Record Open Pub Date : 2020-01-09 eCollection Date: 2020-01-01 DOI:10.1136/vetreco-2019-000339
Hala A R Saed, Hussam M M Ibrahim, Sabry A El-Khodery, Mohamed A Youssef
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引用次数: 4

摘要

目的:研究过渡期荷斯坦奶牛外周血维生素D受体(VDR)、1α-羟化酶(1α- oase)酶和激活正常t细胞表达与分泌的趋化因子(RANTES)的基因表达规律。方法:选取16头荷斯坦奶牛,分别于预产期(EDD)前3周、分娩当天和产后3周采血,采用实时荧光定量PCR技术检测所研究基因的表达谱,并测定葡萄糖、离子钙(Ca)、甲状旁腺激素(PTH)、无机磷(P)、钠(Na)、钾(K)、氯(Cl)和镁(Mg)水平。结果:与EDD前3周相比,VDR基因表达在分娩当天显著降低,在产后3周显著升高。1α- oase基因表达及PTH、K、Na、Cl水平在分娩当日显著升高。Ca水平在分娩当天显著降低,在产后3周显著升高。P水平在分娩当天显著升高,在产后3周显著降低。葡萄糖水平在分娩当天和产后3周显著下降。RANTES基因表达在三个不同时间点间无显著变化。VDR基因表达与1α- oase基因表达、血清葡萄糖、PTH、P、K水平呈负相关,与血清Ca水平呈正相关。1α- oase基因表达与血清PTH、P、K水平呈正相关,与血清Ca水平呈负相关。结论:本研究结果提示监测VDR和1α- oase基因表达作为过渡期代谢变化指标的重要性,提示它们是判断过渡期奶牛健康状况的候选基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Relationship between expression pattern of vitamin D receptor, 1 alpha-hydroxylase enzyme, and chemokine RANTES genes and selected serum parameters during transition period in Holstein dairy cows.

Objectives: The objective of the present study was to evaluate the pattern of genetic expression of vitamin D receptor (VDR), 1 alpha-hydroxylase (1α-OHase) enzyme and chemokine regulated on activation normal T-cell expressed and secreted (RANTES) in peripheral blood of Holstein dairy cows during transition period.

Methods: Blood samples were collected from 16 Holstein dairy cows at 3 weeks prior expected date of delivery (EDD), at the day of parturition, and 3 weeks post-partum for assessment of expression profile of studied genes using real-time PCR and measurement of glucose, ionized calcium (Ca), parathyroid hormone (PTH), inorganic phosphorous (P), sodium (Na), potassium (K), chloride (Cl), and magnesium (Mg) levels.

Results: Compared with 3 weeks prior EDD, VDR gene expression decreased significantly at the day of parturition then increased significantly at 3 weeks post-partum. The genetic expression of 1α-OHase enzyme as well as PTH, K, Na and Cl levels increased significantly at the day of parturition. The Ca level decreased significantly at the day of parturition then increased significantly at 3 weeks post-partum. The P level increased significantly at the day of parturition then decreased significantly at 3 weeks post-partum. Glucose level decreased significantly at the day of parturition and at 3 weeks post-partum. RANTES gene expression showed non-significant changes among the three different time points. The expression of VDR gene had a negative correlation with the expression of 1α-OHase enzyme gene, and serum levels of glucose, PTH, P and K, but had a positive correlation with the serum Ca level. The expression of 1α-OHase enzyme gene had a positive correlation with serum levels of PTH, P and K, but had a negative correlation with the serum Ca level.

Conclusions: Results of the current study indicate the importance of monitoring the genetic expression of VDR and 1α-OHase enzyme as indicators of metabolic changes during transition period, suggesting that they are candidate genes to judge the health status of dairy cows during such period.

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来源期刊
Veterinary Record Open
Veterinary Record Open VETERINARY SCIENCES-
CiteScore
3.00
自引率
0.00%
发文量
25
审稿时长
19 weeks
期刊介绍: Veterinary Record Open is a journal dedicated to publishing specialist veterinary research across a range of topic areas including those of a more niche and specialist nature to that considered in the weekly Vet Record. Research from all disciplines of veterinary interest will be considered. It is an Open Access journal of the British Veterinary Association.
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