胎牛血清中预先存在的HMGB1对小鼠胰腺β细胞生物学的影响。

IF 1.9 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Islets Pub Date : 2020-01-01 Epub Date: 2020-01-14 DOI:10.1080/19382014.2019.1696128

Hyunwoo Chung, Sung Ji Hong, So Won Choi, Chung-Gyu Park

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引用次数: 1

摘要

高迁移率组框1 (HMGB1)可以作为染色质的结构蛋白，同时作为免疫系统的介质。它与胰岛受者的移植物接受度高度相关，是胰岛移植的生物标志物。由于怀疑胎牛血清(FBS)中先前存在的HMGB1会损害小鼠β细胞的活力和功能，我们从胎牛血清中去除HMGB1并研究其影响。有趣的是，从FBS中去除HMGB1似乎不利于培养的小鼠β细胞的活力和功能，这表明FBS中预先存在的HMGB1可能是胰岛细胞培养不可或缺的组成部分。

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The effect of preexisting HMGB1 within fetal bovine serum on murine pancreatic beta cell biology.

High-mobility group box 1 (HMGB1) can act as a structural protein of the chromatin and at the same time as a mediator of the immune system. Its high correlation with the graft acceptance in pancreatic islet recipients makes it a biomarker in islet transplantation. With the suspicion that preexisting HMGB1 in the fetal bovine serum (FBS) would be detrimental to the viability and function of murine beta cells, HMGB1 was removed from FBS and its impact was investigated. Interestingly, the elimination of HMGB1 from FBS seemed unfavorable to the viability and function of cultured murine beta cells, suggesting that the preexisting HMGB1 in the FBS may be an indispensable component of islet cell culture.

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来源期刊

Islets ENDOCRINOLOGY & METABOLISM-

CiteScore

3.30

自引率

4.50%

发文量

审稿时长

>12 weeks

期刊介绍： Islets is the first international, peer-reviewed research journal dedicated to islet biology. Islets publishes high-quality clinical and experimental research into the physiology and pathology of the islets of Langerhans. In addition to original research manuscripts, Islets is the leading source for cutting-edge Perspectives, Reviews and Commentaries. Our goal is to foster communication and a rapid exchange of information through timely publication of important results using print as well as electronic formats.