体外培养大鼠肠道外植体的分离

Current protocols in toxicology Pub Date : 2019-05-23 DOI:10.1002/cptx.79

Anjaney Kothari, Padmavathy Rajagopalan

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引用次数: 4

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Isolating Rat Intestinal Explants for In Vitro Cultures

The small intestine is an important organ primarily involved in digestion of food and absorption of nutrients. In vitro intestinal models are being developed to study this organ in health and disease. Intestinal explants can be used in such investigations since they contain all the major intestinal cell types. A detailed procedure to isolate intestinal explants from the rat jejunum is described. A protocol for culturing them in vitro for up to 24 hr is also provided. © 2019 by John Wiley & Sons, Inc.

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Current protocols in toxicology

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