利用粪便挥发性有机化合物分析法在死前对白尾鹿(Odocoileus virginianus)的化脓性白尾鹿病(CWD)阳性、暴露阴性和已知阴性进行鉴别。

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Christine K Ellis, Steven F Volker, Doreen L Griffin, Kurt C VerCauteren, Tracy A Nichols
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引用次数: 0

摘要

慢性消耗性疾病(CWD)是一种自然发生的传染性、致命性、可传播的海绵状脑病。目前,疾病的确认依赖于死后通过免疫组织化学(IHC)检测咽后淋巴结内侧或大脑中的传染性朊病毒。用这种方法检测活体动物中的 CWD 不切实际,而且 IHC 和其他实验检测方法在检测生物液体或粪便中的低浓度朊病毒方面也不可靠。在此,我们评估了粪便挥发性有机化合物分析在两个阳性鹿场的 CWD 阳性白尾鹿和接触过 CWD 的白尾鹿,以及两个无病鹿场的两组 CWD 阴性鹿之间的鉴别能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Use of faecal volatile organic compound analysis for ante-mortem discrimination between CWD-positive, -negative exposed, and -known negative white-tailed deer (Odocoileus virginianus).

Use of faecal volatile organic compound analysis for ante-mortem discrimination between CWD-positive, -negative exposed, and -known negative white-tailed deer (Odocoileus virginianus).

Use of faecal volatile organic compound analysis for ante-mortem discrimination between CWD-positive, -negative exposed, and -known negative white-tailed deer (Odocoileus virginianus).

Use of faecal volatile organic compound analysis for ante-mortem discrimination between CWD-positive, -negative exposed, and -known negative white-tailed deer (Odocoileus virginianus).

Chronic wasting disease (CWD) is a naturally occurring infectious, fatal, transmissible spongiform encephalopathy of cervids. Currently, disease confirmation relies on post-mortem detection of infectious prions in the medial retropharyngeal lymph nodes or obex in the brain via immunohistochemistry (IHC). Detection of CWD in living animals using this method is impractical, and IHC and other experimental assays are not reliable in detecting low concentrations of prion present in biofluids or faeces. Here, we evaluate the capability of faecal volatile organic compound analysis to discriminate between CWD-positive and -exposed white-tailed deer located at two positive cervid farms, and two groups of CWD-negative deer from two separate disease-free farms.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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