Matthew H Sharp, Matthew W Stefan, Ryan P Lowery, Jacob M Wilson
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On each testing day, subjects consumed either 29.6 g of WPI or WPI + io (whey protein isolate plus Ingredient Optimized Protein<sup>®</sup>) mixed with 236 mL of water. Plasma EAA and branch chain amino acid (BCAA) concentrations were assessed from whole body donated by subjects at pre-consumption and 30, 60, 90, 120, and 180 minutes post consumption.</p><p><strong>Results: </strong>Plasma levels of total EAA concentration was significantly greater in WPI + io at 30, 60, 90, and 120 minutes post consumption (<i>P</i> < .01, <i>P</i> < .001, <i>P</i> < .01, and <i>P</i> < .01, respectively). Plasma levels of total BCAA concentration was significantly greater in WPI + io at 30, 60, 90, and 120 minutes post consumption (<i>P</i> < .01, <i>P</i> < .001, <i>P</i> < .01, and <i>P</i> < .05, respectively) compared with WPI. For leucine, only WPI + io had elevated levels compared with pre-test at 90 minutes post consumption (<i>P</i> < .001).</p><p><strong>Discussion: </strong>Both conditions significantly elevated EAA, BCAA, and leucine from basal levels. However, we conclude that the consumption of the treated WPI significantly raises plasma EAA, BCAA, and leucine to a greater extent compared with WPI with no treatment. Thus, supplementation with WPI that has undergone Ingredient Optimized<sup>®</sup> technology may be highly beneficial for those who partake in regular exercise, elderly individuals, or those affected by a reduced sensitivity to amino acids.</p>","PeriodicalId":19396,"journal":{"name":"Nutrition and Metabolic Insights","volume":"12 ","pages":"1178638819827970"},"PeriodicalIF":2.3000,"publicationDate":"2019-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1178638819827970","citationCount":"3","resultStr":"{\"title\":\"Postprandial Plasma Amino Acid Responses Between Standard Whey Protein Isolate and Whey Protein Isolate Plus Novel Technology.\",\"authors\":\"Matthew H Sharp, Matthew W Stefan, Ryan P Lowery, Jacob M Wilson\",\"doi\":\"10.1177/1178638819827970\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Muscle mass is an important determinant of metabolic health and physical function. 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Plasma EAA and branch chain amino acid (BCAA) concentrations were assessed from whole body donated by subjects at pre-consumption and 30, 60, 90, 120, and 180 minutes post consumption.</p><p><strong>Results: </strong>Plasma levels of total EAA concentration was significantly greater in WPI + io at 30, 60, 90, and 120 minutes post consumption (<i>P</i> < .01, <i>P</i> < .001, <i>P</i> < .01, and <i>P</i> < .01, respectively). Plasma levels of total BCAA concentration was significantly greater in WPI + io at 30, 60, 90, and 120 minutes post consumption (<i>P</i> < .01, <i>P</i> < .001, <i>P</i> < .01, and <i>P</i> < .05, respectively) compared with WPI. For leucine, only WPI + io had elevated levels compared with pre-test at 90 minutes post consumption (<i>P</i> < .001).</p><p><strong>Discussion: </strong>Both conditions significantly elevated EAA, BCAA, and leucine from basal levels. 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引用次数: 3
摘要
背景：肌肉质量是代谢健康和身体功能的重要决定因素。先前已经证明，餐后循环必需氨基酸（EAA）的增加是肌肉蛋白质合成（MPS）的主要刺激因素。本研究研究了2种不同形式的乳清蛋白分离物（WPI）在等热量和等氮条件下的餐后血浆氨基酸（AA）反应，以研究EAA的血浆浓度 = 12） 19岁至32岁 年龄为岁的受试者被招募进行一项随机交叉设计，该设计包括在两个测试日消耗蛋白质补充剂，两个测试日期之间相隔6天的冲洗期。在每个测试日，受试者消耗29.6 g WPI或WPI + io（乳清蛋白分离物加成分优化蛋白®）与236混合 mL水。血浆EAA和支链氨基酸（BCAA）浓度由受试者在食用前和30、60、90、120和180时捐献的全身进行评估 消费后分钟。结果：WPI患者的血浆总EAA浓度明显高于WPI患者 + 30、60、90和120时的io 消耗后分钟数（P P P P P P P P P 讨论：这两种情况都显著提高EAA、BCAA和亮氨酸的基础水平。然而，我们得出的结论是，与未经处理的WPI相比，食用处理过的WPI显著提高了血浆EAA、BCAA和亮氨酸的程度。因此，补充经过Ingredient Optimized®技术的WPI可能对那些经常锻炼的人、老年人或对氨基酸敏感性降低的人非常有益。
Postprandial Plasma Amino Acid Responses Between Standard Whey Protein Isolate and Whey Protein Isolate Plus Novel Technology.
Background: Muscle mass is an important determinant of metabolic health and physical function. It has previously been demonstrated that the postprandial rise in circulating essential amino acids (EAA) acts as the main stimulus for muscle protein synthesis (MPS). This study investigated postprandial plasma amino acid (AA) responses of 2 different forms of whey protein isolate (WPI) with iso-caloric and iso-nitrogenous profiles to investigate plasma concentrations of EAA.
Methods: In all, 12 healthy men (n = 12) between 19 and 32 years of age were recruited for a randomized, cross-over design, which involved consumption of protein supplements on 2 testing days separated by a 6-day washout period between conditions. On each testing day, subjects consumed either 29.6 g of WPI or WPI + io (whey protein isolate plus Ingredient Optimized Protein®) mixed with 236 mL of water. Plasma EAA and branch chain amino acid (BCAA) concentrations were assessed from whole body donated by subjects at pre-consumption and 30, 60, 90, 120, and 180 minutes post consumption.
Results: Plasma levels of total EAA concentration was significantly greater in WPI + io at 30, 60, 90, and 120 minutes post consumption (P < .01, P < .001, P < .01, and P < .01, respectively). Plasma levels of total BCAA concentration was significantly greater in WPI + io at 30, 60, 90, and 120 minutes post consumption (P < .01, P < .001, P < .01, and P < .05, respectively) compared with WPI. For leucine, only WPI + io had elevated levels compared with pre-test at 90 minutes post consumption (P < .001).
Discussion: Both conditions significantly elevated EAA, BCAA, and leucine from basal levels. However, we conclude that the consumption of the treated WPI significantly raises plasma EAA, BCAA, and leucine to a greater extent compared with WPI with no treatment. Thus, supplementation with WPI that has undergone Ingredient Optimized® technology may be highly beneficial for those who partake in regular exercise, elderly individuals, or those affected by a reduced sensitivity to amino acids.
期刊介绍：
Nutrition and Metabolic Insights is a peer-reviewed, open-access online journal focusing on all aspects of nutrition and metabolism. This encompasses nutrition, including the biochemistry of metabolism, exercise and associated physical processes and also includes clinical articles that relate to metabolism, such as obesity, lipidemias and diabetes. It includes research at the molecular, cellular and organismal levels. This journal welcomes new manuscripts for peer review on the following topics: Nutrition, including the biochemistry of metabolism, Exercise and associated physical processes, Clinical articles that relate to metabolism, such as obesity, lipidemias and diabetes, Research at the molecular, cellular and organismal levels, Other areas of interest include gene-nutrient interactions, the effects of hormones, models of metabolic function, macronutrient interactions, outcomes of changes in diet, and pathophysiology.
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