2-氨基-7- deaza -7-丙基-6-乙烯嘌呤修饰RNA交联寡核苷酸的合成。

Q4 Chemistry

Current Protocols in Nucleic Acid Chemistry Pub Date : 2019-06-01 Epub Date: 2019-03-12 DOI:10.1002/cpnc.79

Ken Yamada, Yusuke Abe, Fumi Nagatsugi

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引用次数: 0

摘要

本文介绍了用可交联的2-氨基-7-二氮杂-7-丙基-6-乙烯基嘌呤(ADpVP)修饰2'-OMe-RNA的合成方法及体外交联实验的制备。所有的合成步骤都以中等或较高的产率产生所需的化合物，而不需要昂贵的化学试剂或特定的装置。修饰后的交联活性形式的RNA也可以用常用的反相高效液相色谱纯化，冻干后可在-80℃下保存数天，可用于交联实验。这种交联活性RNA可以有效地与互补RNA以序列特异性的方式形成共价键。©2019 by John Wiley & Sons, Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Synthesis of RNA Crosslinking Oligonucleotides Modified with 2-Amino-7-Deaza-7-Propynyl-6-Vinylpurine.

This article describes procedures to synthesize 2'-OMe-RNA modified with cross-linkable 2-amino-7-deaza-7-propynyl-6-vinylpurine (ADpVP) and preparation of the RNA-crosslinking experiment in vitro. All synthesis steps yield the desired compound in moderate or high yield without expensive chemical reagents or specific devices. The crosslink-active form of modified RNA can also be purified by commonly used reversed-phase HPLC, can be stored at -80°C after lyophilization for a few days, and is ready to use for crosslinking experiments. This crosslink-active RNA can efficiently form covalent bonds with complementary RNA in a sequence-specific manner. © 2019 by John Wiley & Sons, Inc.

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来源期刊

Current Protocols in Nucleic Acid Chemistry Chemistry-Organic Chemistry

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期刊介绍： Published in association with International Society for Nucleosides, Nucleotides & Nucleic Acids (IS3NA) , Current Protocols in Nucleic Acid Chemistry is equally valuable for biotech, pharmaceutical, and academic labs. It is the resource for designing and running successful research projects in the rapidly growing and changing field of nucleic acid, nucleotide, and nucleoside research.