植物细胞生物学中的电子断层扫描

IF 1.8 4区工程技术

Microscopy Pub Date : 2019-02-01 DOI:10.1093/jmicro/dfy133

Marisa S Otegui;Jannice G Pennington

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引用次数: 19

摘要

这篇综述讨论了三维电子显微镜的最新进展，重点是电子断层扫描对分析植物细胞器和细胞过程的贡献。电子断层扫描（ET）方法是基于通过透射电子显微镜（TEM）在不同倾角下对生物样本进行成像。ET可以应用于塑料嵌入样品和冷冻样品。TEM、直接电子检测、自动图像采集和成像处理算法的技术进步使细胞和细胞器的断层重建具有2–7-nm的轴向分辨率。在这篇综述中，我们讨论了ET在植物细胞生物学中的应用，以及通过冷冻ET和其他3D电子显微镜方法对植物细胞成像的新机会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Electron tomography in plant cell biology

This review discusses recent advances in three-dimensional electron microscopy with an emphasis on the contributions of electron tomography to the analysis of plant organelles and cellular processes. Electron tomography (ET) approaches are based on the imaging of a biological specimen at different tilt angles by transmission electron microscopy (TEM). ET can be applied to both plastic-embedded and frozen samples. Technological advancements in TEM, direct electron detection, automated image collection, and imaging processing algorithms allow for 2–7-nm scale axial resolution in tomographic reconstructions of cells and organelles. In this review, we discussed the application of ET in plant cell biology and new opportunities for imaging plant cells by cryo-ET and other 3D electron microscopy approaches.

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来源期刊

Microscopy 工程技术-显微镜技术

自引率

11.10%

发文量

审稿时长

>12 weeks

期刊介绍： Microscopy, previously Journal of Electron Microscopy, promotes research combined with any type of microscopy techniques, applied in life and material sciences. Microscopy is the official journal of the Japanese Society of Microscopy.