分析整合素依赖性粘附

Q3 Biochemistry, Genetics and Molecular Biology

Current Protocols in Cell Biology Pub Date : 2018-10-01 DOI:10.1002/cpcb.69

A. Paul Mould

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引用次数: 7

摘要

在本单元中，描述了整合素依赖性粘附的分析方法。两种主要类型的分析通常用于这种分析。首先是细胞粘附试验。这类检测的一个关键应用是确定哪些整合素介导细胞-底物相互作用;详细列出了适用于此目的的抗体的综合清单。第二种是固相测定，其中使用纯化的整合素和整合素配体。这些检测方法可用于测量整合素对不同配体的表观亲和力和药理学抑制剂的IC50值。咕咕叫。Protoc。细胞生物学。53:9.4.1-9.4.17。©2018 by John Wiley &儿子,Inc。

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Analyzing Integrin-Dependent Adhesion

In this unit, methods for the analysis of integrin-dependent adhesion are described. Two major types of assays are commonly used for this analysis. The first are cell adhesion assays. A key application of this type of assay is to identify which integrin(s) mediate cell-substrate interactions; a comprehensive list of antibodies suitable for this purpose is detailed. The second are solid-phase assays in which purified integrins and integrin ligands are used. These assays can be used, e.g., to measure apparent affinities of integrins for different ligands and IC₅₀ values of pharmacological inhibitors. Curr. Protoc. Cell Biol. 53:9.4.1-9.4.17. © 2018 by John Wiley & Sons, Inc.

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Current Protocols in Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

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期刊介绍： Developed by leading scientists in the field, Current Protocols in Cell Biology is an essential reference for researchers who study the relationship between specific molecules and genes and their location, function and structure at the cellular level. Updated every three months in all formats, CPCB is constantly evolving to keep pace with the very latest discoveries and developments.