Bacterial Biofilm Inhibition in the Development of Effective Anti-Virulence Strategy.

It was observed that bacterial cells inside the biofilm are thousand times more resistant to the conventional antibiotics than the free-living forms [4]. The biofilm antibiotic resistance is correlatable to various mechanisms involved in both cellular and community level, including, at cellular level, enzymatic resistance, chemical modification of the antibiotic target and changes in cell permeability whereas, at biofilm level, the limited penetration of the antibiotic and the presence in the deepest layers of dormant cells intrinsically resistant to conventional antibiotic treatments [5, 6].