芽孢杆菌产外菊粉酶的部分纯化及特性研究。

IF 3.5 Q3 Biochemistry, Genetics and Molecular Biology
R. Ramapriya, A. Thirumurugan, T. Sathishkumar, D.R. Manimaran
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引用次数: 10

摘要

菊粉酶是近年来备受关注的工业食品酶。本研究从3种不同的植物块根土壤样品中分离和筛选了产菊粉酶的8个菌落。在8个产菊粉酶菌落中,选择产量最高的菌落为25.10 U/mL进行进一步研究。经部分16S rRNA基因序列鉴定,产菊粉酶的最佳菌落为Bacillus sp.。粗酶经DEAE - sepacel上硫酸铵沉淀、透析和离子交换层析纯化得到1.9倍纯化,总活性为293 u。纯化后的酶进行了特性研究,发现该酶在30-60 °C下稳定,最适温度为55 °C。酶在pH 3.0 ~ 7.0稳定,最适pH为6.5。菊粉酶的Km和Vmax分别为0.117 mg/mL和4.45 μmol min mg−1,表明其具有较强的亲和性。因此,该酶可广泛用于生产果糖和低聚果糖,而低聚果糖是食品和制药工业的重要原料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Partial purification and characterization of exoinulinase produced from Bacillus sp.

Partial purification and characterization of exoinulinase produced from Bacillus sp.

Partial purification and characterization of exoinulinase produced from Bacillus sp.

Partial purification and characterization of exoinulinase produced from Bacillus sp.

Inulinase are industrial food enzymes which have gained much attention in recent scenario. In this study, Inulinase producing eight bacterial colonies were isolated and screened from three different plant root tubers soil sample. Among 8 inulinase producing colonies, the higher yielding colony was selected with 25.10 U/mL for further studies. The best inulinase producing colony was identified by partial 16S rRNA gene sequence as Bacillus sp. The crude inulinase was purified by using ammonium sulphate precipitation, dialysis and ion exchange chromatography on DEAE – sephacel and obtained 1.9 purification fold with total activity 293 U. The purified enzyme was subjected to characterization studies and it was found to be stable at 30–60 °C and optimum temperature was at 55 °C. The enzyme was stable at pH 3.0–7.0 and optimum pH was at 6.5. The Km and Vmax value for inulinase was found to be 0.117 mg/mL and 4.45 μmol min mg−1 respectively, demonstrate its greater affinity. Hence, this enzyme can be widely used for the production of fructose, and fructooligosaccharides, which are important ingredients in food and pharmaceutical industry.

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来源期刊
Journal of Genetic Engineering and Biotechnology
Journal of Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
5.70
自引率
5.70%
发文量
159
审稿时长
16 weeks
期刊介绍: Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts
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