一种用于脑类器官长期生长和实时成像的芯片方法

Q3 Biochemistry, Genetics and Molecular Biology

Current Protocols in Cell Biology Pub Date : 2018-09-21 DOI:10.1002/cpcb.62

Eyal Karzbrun, Rami Yair Tshuva, Orly Reiner

{"title":"一种用于脑类器官长期生长和实时成像的芯片方法","authors":"Eyal Karzbrun, Rami Yair Tshuva, Orly Reiner","doi":"10.1002/cpcb.62","DOIUrl":null,"url":null,"abstract":"<p>Brain organoids are an emerging technique for studying human neurodevelopment in vitro, with biomedical implications. However, three-dimensional tissue culture poses several challenges, including lack of nutrient exchange at the organoid core and limited imaging accessibility of whole organoids. Here we present a method for culturing organoids in a micro-fabricated device that enables in situ real-time imaging over weeks with efficient nutrient exchange by diffusion. Our on-chip approach offers a means for studying the dynamics of organoid development, cell differentiation, cell cycle, and motion. © 2018 by John Wiley & Sons, Inc.</p>","PeriodicalId":40051,"journal":{"name":"Current Protocols in Cell Biology","volume":"81 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpcb.62","citationCount":"12","resultStr":"{\"title\":\"An On-Chip Method for Long-Term Growth and Real-Time Imaging of Brain Organoids\",\"authors\":\"Eyal Karzbrun, Rami Yair Tshuva, Orly Reiner\",\"doi\":\"10.1002/cpcb.62\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Brain organoids are an emerging technique for studying human neurodevelopment in vitro, with biomedical implications. However, three-dimensional tissue culture poses several challenges, including lack of nutrient exchange at the organoid core and limited imaging accessibility of whole organoids. Here we present a method for culturing organoids in a micro-fabricated device that enables in situ real-time imaging over weeks with efficient nutrient exchange by diffusion. Our on-chip approach offers a means for studying the dynamics of organoid development, cell differentiation, cell cycle, and motion. © 2018 by John Wiley & Sons, Inc.</p>\",\"PeriodicalId\":40051,\"journal\":{\"name\":\"Current Protocols in Cell Biology\",\"volume\":\"81 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-09-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/cpcb.62\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Protocols in Cell Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cpcb.62\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Cell Biology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpcb.62","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 12

摘要

脑类器官是一种新兴的体外研究人类神经发育的技术，具有生物医学意义。然而，三维组织培养面临着一些挑战，包括类器官核心缺乏营养交换和整个类器官的成像可及性有限。在这里，我们提出了一种在微制造设备中培养类器官的方法，该方法可以通过扩散进行有效的营养交换，从而在数周内实现原位实时成像。我们的芯片方法为研究类器官发育、细胞分化、细胞周期和运动的动力学提供了一种手段。©2018 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

An On-Chip Method for Long-Term Growth and Real-Time Imaging of Brain Organoids

Brain organoids are an emerging technique for studying human neurodevelopment in vitro, with biomedical implications. However, three-dimensional tissue culture poses several challenges, including lack of nutrient exchange at the organoid core and limited imaging accessibility of whole organoids. Here we present a method for culturing organoids in a micro-fabricated device that enables in situ real-time imaging over weeks with efficient nutrient exchange by diffusion. Our on-chip approach offers a means for studying the dynamics of organoid development, cell differentiation, cell cycle, and motion. © 2018 by John Wiley & Sons, Inc.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Current Protocols in Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

自引率

0.00%

发文量

期刊介绍： Developed by leading scientists in the field, Current Protocols in Cell Biology is an essential reference for researchers who study the relationship between specific molecules and genes and their location, function and structure at the cellular level. Updated every three months in all formats, CPCB is constantly evolving to keep pace with the very latest discoveries and developments.