[利用DNA条形码技术鉴定鱼肚原种]。

中药材 Pub Date : 2016-09-01

Xuan Cai, Wen Qiu, En-wei Tian, Hong-wei Zhang, Hao-ting Ye, Zhi Chao

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引用次数: 0

摘要

目的:利用DNA条形码技术对广州市场销售的鱼肚原种进行鉴定。方法:对11份鱼鳔标本的线粒体细胞色素C亚基I (coi)基因片段进行扩增，并用自行设计的引物进行测序。构建UPGMA系统发育树进行聚类分析。每个样本的物种来源用生命数据系统条形码(BOLD)提供的识别引擎进行鉴定。结果:自行设计的引物对鱼鳔CO I的扩增和测序均有效，成功率均为100%。BOLD鉴定和UPGMA聚类分析表明，样品来自3科5种鱼类。结论:DNA条形码结合BOLD识别系统可准确识别商品鱼肚的种类来源。

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[Identification of Original Species of Fish Maw by DNA Barcoding].

Objective: To identify the original species of fish maw sold in Guangzhou market by DNA barcoding technology.

Methods: Mitochondrial cytochrome C subunit I (CO I) gene fragment of eleven fish maw samples were amplified and sequenced with the self-designed primers. UPGMA phylogenetic tree were constructed for clustering analysis. The species origin of each sample was identified with the identification engine provided in the Barcode of Life Data Systems (BOLD).

Results: The self-designed primers were effective in fish maw CO I amplification and sequencing, with success rates both of 100%. BOLD identification and UPGMA clustering analysis indicated the fish maw samples were derived from five fish species of three families.

Conclusion: DNA barcoding combined with BOLD identification system can accurately identify the species origin of commercial fish maw.

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来源期刊

中药材

自引率

0.00%

发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.