[丹参遗传连锁图谱的构建]。

中药材 Pub Date : 2016-07-01

Yu-ling Pan, Qi Zhao, Zhen-qiao Song, Jian-hua Wang

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引用次数: 0

摘要

目的:在前人研究的基础上，利用EST-SSR引物构建丹参遗传图谱，为定位与基因相关的重要性状、克隆及分子标记辅助选育丹参新品种搭建平台。方法:利用411条EST-SSR引物进行PCR扩增，筛选两亲本丹参ZH74和BH18杂交F1定位群体的多态性标记。结合以往研究的分子标记数据，Joinmap 4。使用0软件进行地图整合。结果:从两个亲本中共筛选到411EST-SSR引物，扩增出稳定产物328对，获得多态性引物164对。结论:构建了具有150个标记的丹参高密度遗传连锁图谱。

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[Construction of A Genetic Linkage Map in Salvia miltiorrhiza].

Objective: On the basis of previous studies, to construct a genetic map of Salvia miltiorrhiza by using EST-SSR primers, to build a platform for positioning important traits relating to genes, the cloning and molecular marker-assisted in breeding of new varieties of Salvia miltiorrhiza.

Methods: A total of 411 EST-SSR primers were used for PCR amplification to screen polymorphic markers in F1 mapping population derived from a cross between Salvia miltiorrhiza, two parents of which were the cultivars of ZH74 and BH18. Combined with the molecular marker data of previous studies, Joinmap 4. 0 software was used for map integration.

Results: 411EST-SSR primers were screened from two parents, a total of 328 pairs of primers were amplified stable products,164 pairs of polymorphic primers were obtained.

Conclusion: A linkage map of Salvia miltiorrhiza with 150 marker high density genetic is constructed.

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来源期刊

中药材

自引率

0.00%

发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.