不同菌株灵芝漆酶活性比较[j]。

中药材 Pub Date : 2016-07-01

Yu Liu, Jin Lan, Xin-ran Xu, Xiang-dong Chen, Chun-mei Yin

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引用次数: 0

摘要

目的:探讨灵芝菌漆酶活性与生长的关系，筛选高效漆酶菌株。方法:在PDA固体培养基中培养菌丝体，采用ABTS+法测定漆酶活性。采用苯酚硫酸法测定多糖的含量。结果:赤芝早期生长较快，漆酶活性最高(1 476)。64 U/g)，第4天为11。是甜灵芝的37倍。综合分析，赤芝具有显著优势。信书灵芝多糖含量较低;菌株S3生长速度稳定，多糖含量中等。结论:不同品种灵芝的生长速度存在一定差异，漆酶活性与生长有一定的相关性。在筛选菌株中，赤芝是产漆酶最高的菌株。

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[Comparison of Laccase Activity of Different Strains of Ganoderma spp.].

Objective: To explore the relationship between the laccase activity and growth of Ganoderma spp.,to screen the highproducing laccase strains.

Methods: The mycelium was cultured in PDA solid medium,and the laccase activity was measured by ABTS+method. The content of polysaccharide was determined by phenol sulfuric acid method.

Results: Ganoderma gibbosum grew more rapidly in the early stage,and the laccase activity reached maximum( 1 476. 64 U/g) in the 4th day,which was 11. 37 times larger than that of sweet Ganoderma lucidum. Ganoderma gibbosum had the significant advantage after a comprehensive analysis. The polysaccharide content of Ganoderma lucidum of Shinshu was lower; the growth rate of Ganoderma lucidum strain S3 was stable, and the content of polysaccharide was medium.

Conclusion: There are some differences in the growth rate of Ganoderma spp., and there is a certain correlation between the laccase activity and growth. Ganoderma gibbosum is the highest-producing laccase strain among the screening strains.

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来源期刊

中药材

自引率

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发文量

11364

期刊介绍： "Chinese Materia Medica" magazine was founded in 1978. It is a national-level academic journal of Chinese medicine science and technology approved by the State Science and Technology Commission and the Press and Publication Administration for public distribution at home and abroad. It is sponsored by the Chinese Materia Medica Information Center of the State Food and Drug Administration and is publicly distributed at home and abroad. It mainly reports on new technologies, new methods and new achievements in the production, scientific research and business management of Chinese medicine, and serves to promote the production and scientific and technological development of Chinese medicine, improve the output and quality of Chinese medicine, improve business management, and prosper the Chinese medicine industry.