用噬菌体展示法分离硫酸肝素抗体

Q1 Biochemistry, Genetics and Molecular Biology

Current Protocols in Protein Science Pub Date : 2018-08-09 DOI:10.1002/cpps.66

Heungnam Kim, Mitchell Ho

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引用次数: 14

摘要

硫酸乙酰肝素(HS)在发育和疾病中起重要作用。它与许多生长因子、趋化因子和其他已知对细胞生长、运动和分化重要的配体相互作用。然而，由于HS的免疫原性较差，很难在小鼠、兔子或人类中分离出针对HS的抗体。噬菌体展示是一种主要的抗体工程技术，它允许选择免疫原性差或高度保守的抗原的抗体。本方案包含HS抗原制备、与HS结合glypican-3 (GPC3)的人单链Fv (HS20)噬菌体的分离和所选噬菌体抗体的分析的详细步骤。可以想象，本方案中描述的程序可能适用于分离各种HS分子的抗体。©2018 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Isolation of Antibodies to Heparan Sulfate on Glypicans by Phage Display

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Isolation of Antibodies to Heparan Sulfate on Glypicans by Phage Display

Heparan sulfate (HS) plays an important role in development and disease. It interacts with many growth factors, chemokines, and other ligands known to be important for cell growth, motility, and differentiation. However, isolating an antibody to HS in mice, rabbits, or humans is difficult due to the poor immunogenicity of HS. Phage display is a major antibody engineering technology that allows the selection of antibodies for poorly immunogenic or highly conserved antigens. This protocol contains detailed procedures for HS antigen preparation and isolation of a phage displayed human single-chain Fv (HS20) that binds HS on glypican-3 (GPC3), and analysis of the selected phage antibody. It is conceivable that the procedures described in this protocol may be applicable to the isolation of antibodies for a variety of HS molecules. © 2018 by John Wiley & Sons, Inc.

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来源期刊

Current Protocols in Protein Science Biochemistry, Genetics and Molecular Biology-Biochemistry

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期刊介绍： With the mapping of the human genome, more and more researchers are exploring protein structures and functions in living organisms. Current Protocols in Protein Science provides protein scientists, biochemists, molecular biologists, geneticists, and others with the first comprehensive suite of protocols for this growing field.