Sholeh Ghabraei, Behnam Bolhari, Mohammad Marvi Sabbagh, Mahsa Sobhi Afshar
{"title":"三联抗生素糊剂与氢氧化钙混合2%氯己定对粪肠球菌生物膜的抗菌效果比较。","authors":"Sholeh Ghabraei, Behnam Bolhari, Mohammad Marvi Sabbagh, Mahsa Sobhi Afshar","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>The purpose of this in-vitro study was to determine and compare the shortest period needed for a triple antibiotic paste (TAP) and calcium hydroxide (Ca(OH)<sub>2</sub>) plus 2% chlorhexidine (CHX) to eradicate the biofilm of Enterococcus <i>faecalis</i> (EF) from the root canal system.</p><p><strong>Materials and methods: </strong>Sixty-five extracted single-rooted human teeth with straight root canals were selected. The crowns were cut from the cementoenamel junction (CEJ), and canal preparations were done by step-back technique. The smear layer was removed by 17% ethylenediaminetetraacetic acid (EDTA) and 5.25% sodium hypochlorite (NaOCl). Afterwards, the samples were sterilized with gamma ray and were placed inside microtubes for one week. During this week, the teeth were infected with EF. Then, a TAP and Ca(OH)<sub>2</sub> mixed with 2% CHX were inserted into the canals. The roots were cut longitudinally, and dentin chips were collected from the apical part of the roots by a round bur to the depth of 400 μm. The vital bacterial load was assessed by counting the numbers of colony-forming units (CFUs).</p><p><strong>Results: </strong>The paste of Ca(OH)<sub>2</sub> mixed with 2% CHX was able to eradicate the EF biofilm in three days. The TAP was able to eradicate the biofilm of EF in seven days.</p><p><strong>Conclusions: </strong>It seems that Ca(OH)<sub>2</sub> mixed with 2% CHX is more potent than the TAP against EF biofilm.</p>","PeriodicalId":30286,"journal":{"name":"Journal of Dentistry of Tehran University of Medical Sciences","volume":"15 3","pages":"151-160"},"PeriodicalIF":0.0000,"publicationDate":"2018-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079187/pdf/","citationCount":"0","resultStr":"{\"title\":\"Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm.\",\"authors\":\"Sholeh Ghabraei, Behnam Bolhari, Mohammad Marvi Sabbagh, Mahsa Sobhi Afshar\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>The purpose of this in-vitro study was to determine and compare the shortest period needed for a triple antibiotic paste (TAP) and calcium hydroxide (Ca(OH)<sub>2</sub>) plus 2% chlorhexidine (CHX) to eradicate the biofilm of Enterococcus <i>faecalis</i> (EF) from the root canal system.</p><p><strong>Materials and methods: </strong>Sixty-five extracted single-rooted human teeth with straight root canals were selected. The crowns were cut from the cementoenamel junction (CEJ), and canal preparations were done by step-back technique. The smear layer was removed by 17% ethylenediaminetetraacetic acid (EDTA) and 5.25% sodium hypochlorite (NaOCl). Afterwards, the samples were sterilized with gamma ray and were placed inside microtubes for one week. During this week, the teeth were infected with EF. Then, a TAP and Ca(OH)<sub>2</sub> mixed with 2% CHX were inserted into the canals. The roots were cut longitudinally, and dentin chips were collected from the apical part of the roots by a round bur to the depth of 400 μm. The vital bacterial load was assessed by counting the numbers of colony-forming units (CFUs).</p><p><strong>Results: </strong>The paste of Ca(OH)<sub>2</sub> mixed with 2% CHX was able to eradicate the EF biofilm in three days. The TAP was able to eradicate the biofilm of EF in seven days.</p><p><strong>Conclusions: </strong>It seems that Ca(OH)<sub>2</sub> mixed with 2% CHX is more potent than the TAP against EF biofilm.</p>\",\"PeriodicalId\":30286,\"journal\":{\"name\":\"Journal of Dentistry of Tehran University of Medical Sciences\",\"volume\":\"15 3\",\"pages\":\"151-160\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6079187/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Dentistry of Tehran University of Medical Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Dentistry of Tehran University of Medical Sciences","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Comparison of Antimicrobial Effects of Triple Antibiotic Paste and Calcium Hydroxide Mixed with 2% Chlorhexidine as Intracanal Medicaments Against Enterococcus faecalis Biofilm.
Objectives: The purpose of this in-vitro study was to determine and compare the shortest period needed for a triple antibiotic paste (TAP) and calcium hydroxide (Ca(OH)2) plus 2% chlorhexidine (CHX) to eradicate the biofilm of Enterococcus faecalis (EF) from the root canal system.
Materials and methods: Sixty-five extracted single-rooted human teeth with straight root canals were selected. The crowns were cut from the cementoenamel junction (CEJ), and canal preparations were done by step-back technique. The smear layer was removed by 17% ethylenediaminetetraacetic acid (EDTA) and 5.25% sodium hypochlorite (NaOCl). Afterwards, the samples were sterilized with gamma ray and were placed inside microtubes for one week. During this week, the teeth were infected with EF. Then, a TAP and Ca(OH)2 mixed with 2% CHX were inserted into the canals. The roots were cut longitudinally, and dentin chips were collected from the apical part of the roots by a round bur to the depth of 400 μm. The vital bacterial load was assessed by counting the numbers of colony-forming units (CFUs).
Results: The paste of Ca(OH)2 mixed with 2% CHX was able to eradicate the EF biofilm in three days. The TAP was able to eradicate the biofilm of EF in seven days.
Conclusions: It seems that Ca(OH)2 mixed with 2% CHX is more potent than the TAP against EF biofilm.
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