2013~2014年昆明市住院婴儿星状病毒感染的分子流行病学分析

Sida Yang, Yuheng Ren, Lili Deng, Jinghui Yang, Yongying Xiong, Shenrong Jing, Li Li
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引用次数: 0

摘要

目的调查2013 - 2014年昆明市住院婴儿人类星状病毒(HAstV)感染情况及分子流行病学特征。采用逆转录聚合酶链式反应(RT-PCR)对63份腹泻粪便和42份对照组的HAstV感染及基因型进行分析。对一株哈斯特病毒株的全基因组序列进行扩增和测序。63份粪便标本中HAstV阳性率为41.27%(26/63)。哈斯特病毒的主要循环基因型为哈斯特v1。42例对照中仅有1例阳性(2.38%)。通过系统发育分析,确定该菌株的完整基因组序列为HAstV1。这些数据为昆明市婴幼儿病毒性腹泻的控制提供了重要的理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Molecular Epidemiology of Astrovirus Infection Among Hospitalized Infants in Kunming City, China, 2013~2014].

We investigated the infection and molecular-epidemiologic characteristics of human astrovirus (HAstV) of hospitalized infants in Kunming City from the year 2013 to 2014.Infection and genotype of HAstV of 63 samples of diarrheal feces and 42 controls were analyzed by reverse transcription-polymerase chain reaction(RT-PCR).The complete genome sequence of a HAstV strain was amplified and sequenced. The positive rate of HAstV in 63 feces samples was 41.27%(26/63).The main circulating genotype of HAstV was HAstV1.Only 1sample was positive for HAstV in 42controls(2.38%).A complete genome sequence of the HAstV strain was identified as HAstV1 by phylogenetic analyses. These data provide an important theoretical basis for the control of viral diarrhea in infants in Kunming City.

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