[Phylogenetic Analysis of Complete VP1 Sequence of Coxsackievirus B1 in Shandong Province, China ].

To investigate the molecular epidemiological characterization of coxsackieivirus B1(CV-B1),we performed VP1 sequencing on all isolates from acute flaccid paralysis (AFP) surveillance, environmental surveillance and viral meningitis specimens from 1994 to 2015.A total of 53CV-B1 strains were isolated, among which 41 strains,4strains,and 8strains were obtained from AFP surveillance, environmental surveillance and meningitis specimens, respectively. Phylogenetic analysis based on VP1 entire coding region revealed that Shandong CV-B1 strains were segregated into a major cluster alongside with other domestic strains, with no foreign strains existing in this cluster. Foreign strains composed two other exclusive branches.Shandong strains had VP1 nucleotide similarities of 84.4%to 100.0% with each other, and 77.9%to 85.0% with foreign strains. The results presented here demonstrate Chinese CV-B1 strains have great genetic divergence with foreign strains. EV associated surveillance should be reinforced to monitor possible importation of different EV transmission lineages.