大鼠同种异体间充质干细胞的免疫调节作用。

I E Trubitsyna, N A Onishchenko, A V Lyundup, O V Knyazev, A S Gulyaev, O S Vasnev, Z M Abdulatipova, A V Smirnova, Yu M Orlova, G A Drozdova
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引用次数: 0

摘要

近年来,细胞治疗越来越受到人们的关注,这就需要研究同种异体间充质干细胞移植治疗急慢性炎症反应的有效性和安全性。本研究旨在探讨同种异体间充质骨髓基质细胞移植对大鼠腺胃外科创伤愈合的影响。材料与方法:选用Wistar大鼠。大鼠骨髓抽吸液制备细胞移植单核组分。注射第8代和第9代细胞。给药第3天和/或第7天的细胞剂量为3,5h106细胞/ ml两次或5,0h106细胞/ ml两次。第10和17天进行尸检。ELISA法测定血清中促炎性因子il - 1β、tnf - α、IFNy、IL-4的含量。结果与讨论:引言MSCs有助于炎症免疫介质IL1P、TNFa、IFNy的下降,抗炎IL4的增加。质量提高愈合。结论:干细胞的快速疗效可能与血液免疫细胞(巨噬细胞)的形成有关,巨噬细胞产生修复受损组织的物质。它们恢复Th1和Th2之间的平衡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
IMMUNOMODULATORY EFFECT OF ALLOGENEIC MESENCHYMAL STEM CELLS OF RATS.

Introduction: Recently, more and more attracted the attention of cell therapy, which requires a study of the efficacy and safety of allogeneic MSCs transplantation in acute and chronic inflammatory reactions. The aim of our study was to examine the effectiveness of transplantation of allogeneic mesenchymal bone marrow stromal cells for the healing of surgical wounds the glandular stomach in rats.

Material and methods: Using white Wistar rats. Producing cell transplantation mononuclear fraction derived from rat bone marrow aspirate. Injected cells 8 and 9 th passage. The dose of cells administered to 3-th and / or 7-th days 3,5h106 cells / ml twice or 5,0h106 cells / ml dose. Autopsy on day 10-th and 17-th. The serum ELISA determined the content of the pro- and anti-inflammatory cytokines IL1β, TNFα, IFNy, IL-4.

Results and discussion: Introduction MSCs contributed to the decline of the immune mediators of inflammation IL1P, TNFa, IFNy, increase anti-inflammatory IL4. Quality improved healing.

Conclusion: Rapid curative effect of stem cells may be associated with the formation of blood immune cells (macrophages) that produce substances that restore damaged tissue. They restore the balance between Th1 and Th2.

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