[DNA核酸酶与猪链球菌血清9型毒力的关系]。

微生物学报 Pub Date : 2017-04-04

Tang Huanyu, Haiyan Ren, Zongfu Wu, Chengping Lu

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引用次数: 0

摘要

目的:除了猪链球菌血清2型外，猪链球菌血清9型(SS9)也是目前流行的一种血清型和人畜共患病原体。在我们之前的研究中，SS9 DNA核酸酶(SsnA)被认为是一个候选毒力因子。为了明确SsnA对SS9毒力的影响，我们构建了SsnA突变体(ΔssnA)并研究了其生物学功能。方法:在斑马鱼感染模型中评估野生型菌株和ΔssnA的毒力，比较野生型菌株和ΔssnA对HEp-2细胞的粘附率、猪血存活率和酶活性。结果:在斑马鱼感染实验中，ΔssnA 50%致死剂量值比野生型菌株高11.2倍。ΔssnA对HEp-2细胞的粘附率仅为野生菌株的60.61%。ΔssnA在猪血中的存活率降至野生株水平的71.88%。酶活性测定表明，SsnA既能降解线性DNA，也能降解环状DNA。结论:SsnA参与了斑马鱼感染模型中SS9的毒力、对HEp-2细胞的粘附以及猪血中SS9的存活。SsnA确实是SS9的重要毒力因子。

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[Relationship between DNA nuclease and the virulence of Streptococcus suis serotype 9].

Objective: In addition to Streptococcus suis serotype 2, Streptococcus suis serotype 9 (SS9) is also a currently prevalent serotype and a zoonotic pathogen. In our previous study, SS9 DNA nuclease (SsnA) was considered as a candidate virulence factor. To clarify the impact of SsnA on SS9 virulence, we constructed ssnA mutant (ΔssnA) and studied its biological functions.

Methods: We evaluated the virulence of wild type strain and ΔssnA in a zebrafish infection model and compared the adherence rate to HEp-2 cells, the survival rate in pig blood, and enzymatic activity between wild type stain and ΔssnA.

Results: In a zebrafish infection experiment, the 50% lethal dose value of ΔssnA was 11.2-fold higher than that of wild type strain. The adherence rate of ΔssnA to HEp-2 cells was only 60.61% of the wild strain level. The survival rate of ΔssnA in pig blood was declined to 71.88% of wild strain level. The enzymatic activity assay showed that SsnA can degrade both linear and circular DNA.

Conclusion: SsnA contributes to SS9 virulence in a zebrafish infection model, the adherence to HEp-2 cells, and the survival in pig blood. SsnA is indeed an essential virulence factor for SS9.

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来源期刊

微生物学报

自引率

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发文量

7960

期刊介绍： Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.