Zahra Karimi, Ali Ahmadi, Ali Najafi, Reza Ranjbar
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引用次数: 28
摘要
简介:CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats，群集规则间隔短回文重复序列)基因座作为一种新颖的、可应用于原核生物基因组的区域，在后基因组学时代受到了极大的关注。方法:这些独特区域在不同病原菌中数量和序列组成不同，可作为分子流行病学和基因分型研究的合适候点。结果:此外，CRISPR基因座的排列结构(与可变序列间隔的几个独特重复序列)和相关cas基因作为一个活跃的原核免疫系统，可抵抗病毒复制和偶联元件。这一特性可作为生物工程研究中RNA编辑的工具。结论:本文综述了CRISPR阵列在基因工程和细菌基因分型研究中的历史、性质和潜在应用。
Bacterial CRISPR Regions: General Features and their Potential for Epidemiological Molecular Typing Studies.
Introduction: CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) loci as novel and applicable regions in prokaryotic genomes have gained great attraction in the post genomics era.
Methods: These unique regions are diverse in number and sequence composition in different pathogenic bacteria and thereby can be a suitable candidate for molecular epidemiology and genotyping studies. Results:Furthermore, the arrayed structure of CRISPR loci (several unique repeats spaced with the variable sequence) and associated cas genes act as an active prokaryotic immune system against viral replication and conjugative elements. This property can be used as a tool for RNA editing in bioengineering studies.
Conclusion: The aim of this review was to survey some details about the history, nature, and potential applications of CRISPR arrays in both genetic engineering and bacterial genotyping studies.
期刊介绍：
The Open Microbiology Journal is a peer-reviewed open access journal which publishes research articles, reviews/mini-reviews, case studies, guest edited thematic issues and short communications/letters covering theoretical and practical aspects of Microbial systematics, evolutionary microbiology, immunology, virology, parasitology , bacteriology, mycology, phycology, protozoology, microbial ecology, molecular biology, microbial physiology, biochemistry, microbial pathogenesis, host-microbe interaction, systems microbiology, synthetic microbiology, bioinformatics. The Open Microbiology Journal , a peer-reviewed journal, is an important and reliable source of current information on developments in the field. The emphasis will be on publishing quality papers rapidly and freely available to researchers worldwide.
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