[东北水稻洪水中噬藻体psbA的遗传多样性]。

微生物学报 Pub Date : 2017-01-04

Ruiyong Jing, Kun Cao, Junjie Liu, Judong Liu, Jian Jin, Xiaobing Liu, Guanghua Wang

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引用次数: 0

摘要

目的:研究东北稻田洪水中噬藻体psbA的遗传多样性及其系统发育位置，为研究噬藻体生态学提供科学依据。方法:采用膜分离富集法、pcr克隆测序法对东北水稻洪水中噬藻体psbA的多样性进行研究。结果:共获得17个噬藻体psbA序列。系统发育分析发现新的噬藻体。与日本稻田洪水、海洋和湖泊相比，东北稻田洪水的psbA基因组合存在显著差异。结论:本文首次采用pcr -克隆-测序技术，利用psbA分子标记研究了东北水稻洪水中噬藻体的遗传多样性。在东北水稻洪水中发现了一种新的噬藻体psbA组装体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

本刊更多论文

[Genetic diversity of psbA of cyanophage from paddy floodwater in northeast China].

Objective: To provide scientific data for studying the ecology of cyanophage, we studied the genetic diversity of psbA of cyanophage from paddy floodwater in northeast China and its phylogenetic positions.

Methods: Membrane separation and concentration of cyanophage, PCR-cloning-sequencing were applied to study the diversity of psbA of cyanophage from paddy floodwater in northeast China.

Results: In total 17 psbA sequences of cyanophage were obtained. Novel cyanophages were found by phylogenetic analysis. Compared to those of Japanese paddy floodwater, marine and lakes, psbA gene assemblage of paddy floodwater in northeast China was significantly different.

Conclusion: This is the first report to study genetic diversity of cyanophage from paddy floodwater in northeast China with a molecular marker of psbA by PCR-cloning-sequencing. The novel psbA assembly of cyanophage was found in paddy floodwater in northeast China.

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来源期刊

微生物学报

自引率

0.00%

发文量

7960

期刊介绍： Acta Microbiologica Sinica(AMS) is a peer-reviewed monthly (one volume per year)international journal,founded in 1953.It covers a wide range of topics in the areas of general and applied microbiology.The journal publishes original papers,reviews in microbiological science,and short communications describing unusual observations. Acta Microbiologica Sinica has been indexed in Index Copernicus (IC),Chemical Abstract (CA),Excerpt Medica Database (EMBASE),AJ of Viniti (Russia),Biological Abstracts (BA),Chinese Science Citation Database (CSCD),China National Knowledge Infrastructure(CNKI),Institute of Scientific and Technical Information of China(ISTIC),Chinese Journal Citation Report(CJCR),Chinese Biological Abstracts,Chinese Pharmaceutical Abstracts,Chinese Medical Abstracts and Chinese Science Abstracts.