高血糖小鼠模型中促炎细胞因子产生的流式细胞术分析。

Q1 Agricultural and Biological Sciences
Muhaimin Rifa I, Mulya D Wahyuningsih, Noviana D Lestari, Aris Soewondo, Mansur Ibrahim, Hideo Tsuboi
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引用次数: 1

摘要

目的:促炎细胞因子的急剧升高已成为一些退行性疾病的严重问题。本研究旨在探讨樱桃叶提取物(CLE)对高血糖小鼠模型中促炎细胞因子产生的影响。最近的一项专利提到,芒tinga calabura是一种含类黄酮的植物,具有补胃、开胃、助消化(CN 201611138374)和胃胀(CN 106071017a)的作用。这种神奇植物的功效激发了这个实验的灵感。方法:采用高脂饮食和20%果糖饮料诱导高血糖。给高血糖小鼠以420、700和28000 mg/kg BW剂量口服CLE,持续两周。治疗后,用流式细胞术分析脾细胞的促炎分子。结果:本研究显示,CLE可降低IL-6、NF-κB、TNF-α、IFN γ等促炎分子的产生。在高血糖小鼠中,我们发现CD68+IL-6+、NK1.1+IL- 6+、B220+NFκB+、CD4+TNF-α+、CD4+IFN γ +与未加操作的对照组相比显著升高(p < 0.05)。给药420、700、2800 mg/kg BW后,促炎分子水平显著降低(p < 0.05),达到正常生理水平。结论:CLE可抑制高血糖小鼠模型中的促炎细胞因子,提示该草药可能是未来退行性疾病或涉及细胞活化的疾病的潜在治疗干预策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Flow Cytometric Analysis of Pro-inflammatory Cytokine Production in Hyperglycemic Mouse Model.

Objective: The sharp increase of pro-inflammatory cytokine becomes a severe problem in some degenerative diseases. This study aimed to investigate the effect of Cherry Leaf Extract (CLE) on pro-inflammatory cytokine production in hyperglycaemic mouse model. A recent patent mentioned that Muntinga calabura is a flavonoid-containing plant which is capable of reinforcing the stomach, appetizing, helping digestion (CN 201611138374), and improving stomach distention (CN106071017A). The efficacy of this miracle plant has inspired this experiment.

Methods: In this study, we induced hyperglycemia with high-fat diet and 20% fructose drink. Hyperglycemic mice were orally administered with CLE with the doses 420, 700, and 28000 mg/kg BW for two weeks. After treatment, the pro-inflammatory molecule was analyzed by flow cytometry from splenic cells.

Results: This study showed that CLE decreased the production of pro-inflammatory molecules such as IL-6, NF-κB, TNF-α, and IFN γ. In hyperglycemic mice, we found CD68+IL-6+, NK1.1+IL- 6+, B220+NFκB+, CD4+TNF-α+, and CD4+IFN γ + increased significantly (p < 0.05) compared to control mice without manipulation. After treatment with 420, 700, or 2800 mg/kg BW of CLE, the pro-inflammatory molecules decreased significantly (p < 0.05), and reached a normal physiology level.

Conclusion: CLE can suppress pro-inflammatory cytokines in hyperglycemic mouse model, suggesting this medicinal herb may be beneficial as a potential strategy for future therapeutic interventions in degenerative diseases or a disease involving cell activation.

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来源期刊
Recent patents on food, nutrition & agriculture
Recent patents on food, nutrition & agriculture Agricultural and Biological Sciences-Agronomy and Crop Science
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