Alexander M Wathne, Hanne Devle, Carl Fredrik Naess-Andresen, Dag Ekeberg
{"title":"气相色谱-质谱联用法测定病毒螺旋体、双头螺旋体和褐螺旋体的脂肪酸含量。","authors":"Alexander M Wathne, Hanne Devle, Carl Fredrik Naess-Andresen, Dag Ekeberg","doi":"10.1155/2018/3679247","DOIUrl":null,"url":null,"abstract":"<p><p>Fatty acid (FA) profiles of the species <i>Tettigonia viridissima</i>, <i>Chorthippus biguttulus</i>, and <i>Chorthippus brunneus</i> were determined and quantitated. Extracted lipids were derivatized into FA methyl esters (FAMEs) prior to analysis by GC-MS. A total of 37 different FAs were identified in <i>T. viridissima</i>, yielding a total FA content of 10.4 g/100 g of dry matter. The contents of saturated FAs, monounsaturated FAs, and polyunsaturated FAs were 31.1, 35.9, and 33.0%, respectively. Lipids from <i>T. viridissima</i> were also fractioned into neutral lipids, free fatty acids, and polar lipids by offline solid phase extraction. For <i>C. brunneus</i> and <i>C. biguttulus</i>, 33 FAs were identified, yielding a total FA content of 6.14 g/100 g of dry matter. SFAs, MUFAs, and PUFAs, respectively, constituted 32.7, 25.1, and 42.1% of the total FA content. The contents of MUFAs, PUFAs, <i>n</i>-3 FAs, and <i>n</i>-6 FAs of each species, and the <i>n</i>-6/<i>n</i>-3 ratio, were subsequently discussed.</p>","PeriodicalId":16274,"journal":{"name":"Journal of Lipids","volume":"2018 ","pages":"3679247"},"PeriodicalIF":5.9000,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/3679247","citationCount":"4","resultStr":"{\"title\":\"Identification and Quantification of Fatty Acids in <i>T. viridissima</i>, <i>C. biguttulus</i>, and <i>C. brunneus</i> by GC-MS.\",\"authors\":\"Alexander M Wathne, Hanne Devle, Carl Fredrik Naess-Andresen, Dag Ekeberg\",\"doi\":\"10.1155/2018/3679247\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Fatty acid (FA) profiles of the species <i>Tettigonia viridissima</i>, <i>Chorthippus biguttulus</i>, and <i>Chorthippus brunneus</i> were determined and quantitated. Extracted lipids were derivatized into FA methyl esters (FAMEs) prior to analysis by GC-MS. A total of 37 different FAs were identified in <i>T. viridissima</i>, yielding a total FA content of 10.4 g/100 g of dry matter. The contents of saturated FAs, monounsaturated FAs, and polyunsaturated FAs were 31.1, 35.9, and 33.0%, respectively. Lipids from <i>T. viridissima</i> were also fractioned into neutral lipids, free fatty acids, and polar lipids by offline solid phase extraction. For <i>C. brunneus</i> and <i>C. biguttulus</i>, 33 FAs were identified, yielding a total FA content of 6.14 g/100 g of dry matter. SFAs, MUFAs, and PUFAs, respectively, constituted 32.7, 25.1, and 42.1% of the total FA content. The contents of MUFAs, PUFAs, <i>n</i>-3 FAs, and <i>n</i>-6 FAs of each species, and the <i>n</i>-6/<i>n</i>-3 ratio, were subsequently discussed.</p>\",\"PeriodicalId\":16274,\"journal\":{\"name\":\"Journal of Lipids\",\"volume\":\"2018 \",\"pages\":\"3679247\"},\"PeriodicalIF\":5.9000,\"publicationDate\":\"2018-02-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1155/2018/3679247\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Lipids\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2018/3679247\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2018/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Lipids","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2018/3679247","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2018/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Identification and Quantification of Fatty Acids in T. viridissima, C. biguttulus, and C. brunneus by GC-MS.
Fatty acid (FA) profiles of the species Tettigonia viridissima, Chorthippus biguttulus, and Chorthippus brunneus were determined and quantitated. Extracted lipids were derivatized into FA methyl esters (FAMEs) prior to analysis by GC-MS. A total of 37 different FAs were identified in T. viridissima, yielding a total FA content of 10.4 g/100 g of dry matter. The contents of saturated FAs, monounsaturated FAs, and polyunsaturated FAs were 31.1, 35.9, and 33.0%, respectively. Lipids from T. viridissima were also fractioned into neutral lipids, free fatty acids, and polar lipids by offline solid phase extraction. For C. brunneus and C. biguttulus, 33 FAs were identified, yielding a total FA content of 6.14 g/100 g of dry matter. SFAs, MUFAs, and PUFAs, respectively, constituted 32.7, 25.1, and 42.1% of the total FA content. The contents of MUFAs, PUFAs, n-3 FAs, and n-6 FAs of each species, and the n-6/n-3 ratio, were subsequently discussed.
期刊介绍：
Journal of Lipids is a peer-reviewed, Open Access journal that publishes original research articles and review articles related to all aspects of lipids, including their biochemistry, synthesis, function in health and disease, and nutrition. As an interdisciplinary journal, Journal of Lipids aims to provide a forum for scientists, physicians, nutritionists, and other relevant health professionals.
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