在阿拉伯联合酋长国使用DNA条形码检测不同草药植物产品中的掺假:概念和验证的证明。

Q1 Agricultural and Biological Sciences
Kareem A Mosa, Sameh Soliman, Ali El-Keblawy, Muna A Ali, Hessa A Hassan, Aysha A B Tamim, Moza M Al-Ali
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引用次数: 10

摘要

背景:市售的草药和药用植物产品容易被其他未标记或不需要的材料替代或污染。这将降低产品的质量,并可能导致中毒和过敏。方法:DNA条形码技术是一种在物种水平上对植物材料进行鉴定的分子技术,通过对细胞核或细胞器基因组的短段标准化基因序列进行简单、快速、准确和经济的测序。本研究的目的是应用DNA条形码来调查阿联酋市场上市售草药和药用植物产品的真实性。总共分析了30个样品,涵盖了六种不同的草药产品(百里香、小豆豉、八角茴香、罗勒、姜黄和生姜),以新鲜和干燥的形式获得。提取DNA,扩增(rbcL)、(matK)和(ITS) 3个条形码位点,BLAST测序分析。结果:在扩增效率方面,rbcL是最适合的物种鉴定标记,扩增成功率为75%,ITS次之,扩增成功率为66.67%,matK最低,扩增成功率为18.52%。在姜粉和干百里香叶两个样品中检出掺假。掺假物主要来自小麦属和稻谷属。结论:本报告的结果为DNA条形码技术在商业植物产品识别中是有效的提供了证据。因此,它可以被认为是一种快速、有效和可靠的方法来检测阿联酋市场上植物性产品中的掺假。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Using DNA Barcoding to Detect Adulteration in Different Herbal Plant- Based Products in the United Arab Emirates: Proof of Concept and Validation.

Background: Commercially available herbal and medicinal plants-based products are susceptible to substitution or contamination with other unlabeled or undesired materials. This will reduce the quality of the product, and may lead to intoxication and allergy.

Methods: DNA barcoding is a molecular technology that allows the identification of plant materials at the species level, by sequencing short stretches of standardized gene sequences from nuclear or organelle genome in an easy, rapid, accurate and cost-effective manner. The aim of this research is to apply DNA barcoding to investigate the authenticity of commercially available herbal and medicinal plant-based products within the UAE markets. A total of 30 samples were analyzed, covering six different herbal products (thyme, cardamom, anise, basil, turmeric, and ginger), obtained in fresh and dried forms. DNA was extracted and three barcode loci including (rbcL), (matK) and (ITS) were amplified, sequenced and analyzed by BLAST.

Results: In terms of amplification efficiency, the results suggest that rbcL is the most suitable marker for species identification giving 75% of successful amplification, followed by ITS with 66.67%, whereas matK had the lowest with 18.52%. Adulteration was detected in two samples, ginger powder and dry thyme leave samples. The adulterants were from Triticum and Oryza genera.

Conclusion: Clearly, the results from this report provide evidence that DNA barcoding technique is efficient in the recognition of commercial plant products. Thus, it can be considered as a fast, effective, and reliable method to detect adulteration in plant-based products in the UAE market.

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来源期刊
Recent patents on food, nutrition & agriculture
Recent patents on food, nutrition & agriculture Agricultural and Biological Sciences-Agronomy and Crop Science
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