猪繁殖与呼吸综合征病毒:繁殖与定量

Zexu Ma, Liping Yang, Yan-Jin Zhang
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引用次数: 3

摘要

猪繁殖与呼吸综合征病毒(PRRSV)是病毒科动脉病毒科的一员。PRRSV是一种包膜单链阳性RNA病毒,基因组长度约为15kb。对于PRRSV的体外繁殖,MARC-145细胞系是实验室环境中最常用的细胞系。已经建立了许多PRRSV株的感染性cDNA克隆,从中可以恢复这些病毒。PRRSV滴定通常在MARC-145细胞中进行。PRRSV RNA拷贝数可以通过逆转录和实时PCR来评估。本文介绍了PRRSV繁殖、从感染性cDNA克隆中恢复病毒和定量的方法。©2018 by John Wiley &儿子,Inc。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Porcine Reproductive and Respiratory Syndrome Virus: Propagation and Quantification

Porcine reproductive and respiratory syndrome virus (PRRSV) is a member of the family Arteriviridae, order Nidovirale. PRRSV is an enveloped, single-stranded, positive-sense RNA virus with a genome around 15 kb in length. For propagation of PRRSV in vitro, the MARC-145 cell line is the most often used in a laboratory setting. Infectious cDNA clones of many PRRSV strains have been established, from which these viruses can be recovered. PRRSV titration is generally done in MARC-145 cells. PRRSV RNA copy numbers can be assessed by reverse transcription and real-time PCR. Here, protocols for PRRSV propagation, virus recovery from infectious cDNA clones, and quantification are presented. © 2018 by John Wiley & Sons, Inc.

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来源期刊
Current Protocols in Microbiology
Current Protocols in Microbiology Immunology and Microbiology-Parasitology
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期刊介绍: Current Protocols in Microbiology provides detailed, step-by-step instructions for analyzing bacteria, animal and plant viruses, fungi, protozoans and other microbes. It offers updated coverage of emerging technologies and concepts, such as biofilms, quorum sensing and quantitative PCR, as well as proteomic and genomic methods. It is the first comprehensive source of high-quality microbiology protocols that reflects and incorporates the new mandates and capabilities of this robust and rapidly evolving discipline.
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