口服镁补充剂对新诊断的高血压前期个体TRPM6、TRPM7和SLC41A1转录的影响一项随机、双盲、安慰剂对照试验。

IF 1.5 4区 医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Mariana Rodríguez-Ramírez, Martha Rodríguez-Morán, Miguel A Reyes-Romero, Fernando Guerrero-Romero
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引用次数: 10

摘要

镁离子转运体对镁的内流和外排的严格调节似乎在血压的调节中起重要作用。因此,我们评估了口服镁补充剂对高血压前期(preHTN)患者TRPM6、TRPM7和SLC41A1转录的影响。为此,我们进行了一项随机、双盲、安慰剂对照试验,在4个月的时间里,比较了18名口服乳酸镁(360毫克元素镁)的患者和18名服用安慰剂的患者。排除标准为高血压或血压正常、糖尿病、饮酒、慢性腹泻、使用利尿剂、补充镁、肾功能下降。RT-qPCR对TRPM6、TRPM7和SLC41A1进行转录分析,获得富白细胞血浆,使用Direct-zol™RNA MiniPrep (zimo)试剂盒分离总RNA。白细胞trpm6mrna相对表达量显著升高(2.1±1.37和0.8±0.4,P
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of oral magnesium supplementation on the transcription of TRPM6, TRPM7, and SLC41A1 in individuals newly diagnosed of pre-hypertension. A randomized, double-blind, placebo-controlled trial.

A stringent regulation of influx and efflux of magnesium by cation transporters seems to play an important role in the regulation of blood pressure (BP). With this regard, we evaluate the effect of oral magnesium supplementation on the transcription of TRPM6, TRPM7, and SLC41A1, in individuals with incident pre-hypertension (preHTN). For such purpose, we conducted a randomized, double-blind, placebo-controlled trial that compared 18 individuals who received oral magnesium lactate (360 mg elemental magnesium) versus 18 individuals who received placebo, during 4 months. Diagnosis of hypertension or normal BP, diabetes, alcohol intake, chronic diarrhea, use of diuretics, intake of magnesium supplementation, and reduced renal function were exclusion criteria. Regarding the transcription analysis of TRPM6, TRPM7, and SLC41A1 using RT-qPCR, leukocyte-rich plasma was obtained and total RNA was isolated with the kit Direct-zol™ RNA MiniPrep (Zymo). The leukocyte TRPM6 mRNA relative expression showed a significant increase (2.1 ± 1.37 and 0.8 ± 0.4, P<0.05), whereas the mRNA relative expression of both leukocyte TRPM7 (0.8 ± 1.1 and 0.9 ± 0.6, pNS) and SLC41A1 (0.9 ± 1.0 and 0.7 ± 0.6, pNS) showed no significant differences, between the magnesium and placebo groups, respectively. Oral magnesium supplementation increases the leukocyte TRPM6 mRNA relative expression, in subjects with new diagnosis of preHTN.

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来源期刊
Magnesium research
Magnesium research 医学-内分泌学与代谢
CiteScore
3.50
自引率
9.40%
发文量
6
审稿时长
>12 weeks
期刊介绍: Magnesium Research, the official journal of the international Society for the Development of Research on Magnesium (SDRM), has been the benchmark journal on the use of magnesium in biomedicine for more than 30 years. This quarterly publication provides regular updates on multinational and multidisciplinary research into magnesium, bringing together original experimental and clinical articles, correspondence, Letters to the Editor, comments on latest news, general features, summaries of relevant articles from other journals, and reports and statements from national and international conferences and symposiums. Indexed in the leading medical databases, Magnesium Research is an essential journal for specialists and general practitioners, for basic and clinical researchers, for practising doctors and academics.
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