淋巴细胞因子在实验性乳腺癌Wistar大鼠肿瘤发生及有效治疗中的作用。

A F Poveshchenko, O V Kazakov, N B Orlov, O V Poveshchenko, I I Kim, N A Bondarenko, I G Solovieva, D N Strunkin, A V Kabakov, T V Rayter, A P Lykov, S S Bogachev, E A Pokushalov, V I Konenkov
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引用次数: 0

摘要

本文的目的是检查淋巴细胞因子的水平参与乳腺癌的发病机制。方法:采用Wistar品种n-甲基-n -亚硝基脲诱导大鼠乳腺癌。有些动物单独接受手术或化疗(环磷酰胺、甲氨蝶呤、5-氟尿嘧啶)。一些动物将两种疗法结合起来,还有一组单独的给药化疗添加了Panagene拖拽呈现片段化的DNA。研究淋巴检测系统Bio-Plex Pro大鼠细胞完整性24-Plex Assay (Bio-Rad, USA)中细胞因子的浓度。结果:乳腺癌大鼠IL-1b、IL-2、IL-4、IL-6、IL-7、IL-12、IL-13、IL-17A、MIP-1a、MIP-3a、RANTES、TNF-a、MCP-1等细胞因子含量显著高于正常小鼠。手术切除肿瘤导致淋巴中促炎细胞因子含量显著降低。对完整动物肿瘤切除后淋巴细胞因子含量的比较性能研究显示,对照组小鼠淋巴细胞因子IL-10、IL-18、GRO / KC、RANTES含量显著升高。化疗导致大鼠乳腺癌淋巴中IL-1b、IL-4、IL-6、IL-7、IL-10、MIP-1a、MIP-3a、RANTES含量显著降低。化疗与Panagene给药后淋巴手术动物细胞因子含量的比较研究显示,给药后淋巴中IL-5、IL-6、IL-7、IL-10、IL-13、IL- 17a、IL- 18、GRO / KC、IFNg、MIP-3a等大部分细胞因子含量指标均升高。结论:通过对淋巴Wistar大鼠细胞因子的比较研究发现,细胞因子的含量与诱导乳腺癌的治疗方式有关。淋巴细胞因子水平可以作为肿瘤生长的诊断标准,也可以作为乳腺癌治疗效果和转移风险的预测指标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lymph cytokines as markers oncogenesis and effective treatment of experimental breast cancer Wistar rat.

The purpose of this paper is to examine the levels of cytokines in the lymph involved in the pathogenesis of breast cancer.

Methods: Breast cancer was induced by introducing n-methyl-N-nitrosourea rats Wistar breed. Some of the animals subjected to surgery alone or chemotherapy alone (cyclophosphamide, methotrexate, 5-fluorouracil). Some animals combine both types of therapy, as well as a separate group to the administration of chemotherapy added Panagene drag presenting a fragmented DNA. To investigate the concentration of cytokines used in lymph test system Bio-Plex Pro Rat Cytokoness 24-Plex Assay (Bio-Rad, USA).

Results: In rats with breast cancer content of most studied cytokines such as, IL-1b, IL-2, IL-4, IL-6, IL-7, IL-12, IL-13, IL-17A, MIP-1a, MIP-3a, RANTES, TNF-a, MCP-1 was significantly higher than in intact animals. Surgical removal of the tumor resulted in a significant decrease in the content in the lymph as a pro-inflammatory cytokine. Comparative performance study cytokine content in the lymph after tumor removal from intact animals showed that the content of cytokines such as IL-10, IL-18, GRO / KC, RANTES were significantly higher in the control animals group. Conducting chemotherapy has led to a significant decrease in the content of IL-1b, IL-4, IL-6, IL-7, IL-10, MIP-1a, MIP-3a, RANTES in rat breast cancer lymph. Comparative study of cytokine content in the lymph operated animals after the administration of chemotherapy and Panagene revealed that most of the content indicators cytokines such as IL-5, IL-6, IL-7, IL-10, IL-13, IL-17A, IL- 18, GRO / KC, IFNg, MIP-3a in the lymph was higher after administration of the drug Panagene.

Conclusion: In a comparative study cytokine profile lymph Wistar rats found that cytokine content depended on the therapy in animals with induced breast cancer. Lymph cytokine levels may serve as a diagnostic criterion for tumor growth, as well as the predictor of the effectiveness of the therapy and the risk of metastasis of breast cancer.

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