Angela Saupe, Birgit Edel, Wolfgang Pfister, Bettina Löffler, Ralf Ehricht, Jürgen Rödel
{"title":"由产志贺毒素2的大肠杆菌O8: H19引起的急性腹泻。","authors":"Angela Saupe, Birgit Edel, Wolfgang Pfister, Bettina Löffler, Ralf Ehricht, Jürgen Rödel","doi":"10.1099/jmmcr.0.005099","DOIUrl":null,"url":null,"abstract":"<p><p><b>Introduction.</b> Identification of non-O157 Shiga-toxin-producing <i>Escherichia coli</i> (STEC) infections may be underestimated in microbiological diagnosis. <b>Case presentation.</b> A 58-year-old woman developed diarrhoea with watery and subsequently mucous stool. Initial multiplex PCR testing revealed a positive result for <i>stx<sub>2</sub></i> . Culture isolation of a STEC was successful only after repeated inoculation of chromogenic <i>E. coli</i> media. Molecular characterization was performed and identified the isolate as <i>stx</i><sub>2e</sub>-positive STEC of serotype O8 : H19. The strain harboured <i>lpfA</i>, but not <i>eae</i>. <b>Conclusion.</b> This case highlights the usefulness of initial multiplex PCR for diagnosis of non-O157 STEC infection.</p>","PeriodicalId":73559,"journal":{"name":"JMM case reports","volume":"4 6","pages":"e005099"},"PeriodicalIF":0.0000,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5630968/pdf/","citationCount":"10","resultStr":"{\"title\":\"Acute diarrhoea due to a Shiga toxin 2e-producing <i>Escherichia coli</i> O8 : H19.\",\"authors\":\"Angela Saupe, Birgit Edel, Wolfgang Pfister, Bettina Löffler, Ralf Ehricht, Jürgen Rödel\",\"doi\":\"10.1099/jmmcr.0.005099\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Introduction.</b> Identification of non-O157 Shiga-toxin-producing <i>Escherichia coli</i> (STEC) infections may be underestimated in microbiological diagnosis. <b>Case presentation.</b> A 58-year-old woman developed diarrhoea with watery and subsequently mucous stool. Initial multiplex PCR testing revealed a positive result for <i>stx<sub>2</sub></i> . Culture isolation of a STEC was successful only after repeated inoculation of chromogenic <i>E. coli</i> media. Molecular characterization was performed and identified the isolate as <i>stx</i><sub>2e</sub>-positive STEC of serotype O8 : H19. The strain harboured <i>lpfA</i>, but not <i>eae</i>. <b>Conclusion.</b> This case highlights the usefulness of initial multiplex PCR for diagnosis of non-O157 STEC infection.</p>\",\"PeriodicalId\":73559,\"journal\":{\"name\":\"JMM case reports\",\"volume\":\"4 6\",\"pages\":\"e005099\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-06-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5630968/pdf/\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"JMM case reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1099/jmmcr.0.005099\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2017/6/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"JMM case reports","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1099/jmmcr.0.005099","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2017/6/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
Acute diarrhoea due to a Shiga toxin 2e-producing Escherichia coli O8 : H19.
Introduction. Identification of non-O157 Shiga-toxin-producing Escherichia coli (STEC) infections may be underestimated in microbiological diagnosis. Case presentation. A 58-year-old woman developed diarrhoea with watery and subsequently mucous stool. Initial multiplex PCR testing revealed a positive result for stx2 . Culture isolation of a STEC was successful only after repeated inoculation of chromogenic E. coli media. Molecular characterization was performed and identified the isolate as stx2e-positive STEC of serotype O8 : H19. The strain harboured lpfA, but not eae. Conclusion. This case highlights the usefulness of initial multiplex PCR for diagnosis of non-O157 STEC infection.
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