主要免疫细胞类型免疫能力的CyTOF测量

Q1 Health Professions
Priyanka B. Subrahmanyam, Holden T. Maecker
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引用次数: 17

摘要

免疫系统在多种疾病中的核心作用越来越受到重视。癌症免疫疗法是最近取得巨大成功的一个领域,尽管并非所有患者都能从中受益。与此同时,最近的研究强调了人类免疫系统的异质性。尽管存在这种异质性,我们在临床实践中并没有常规测量免疫能力,也没有一致的健康免疫功能测定方法。使用细胞计数技术(CyTOF),我们可以同时检测到约40种标记物,以鉴定各种细胞亚群,并通过细胞因子、细胞毒性和激活标记物的表达来确定它们的功能。这可以帮助评估“免疫能力”,促进更好地实施免疫疗法,无论是在特定疾病环境中,还是最终作为健康受试者的预后工具。在这里,我们介绍了这一分析背后的概念,并提供了一个方案,我们已经成功地实施,以确定可能的预测免疫治疗结果的生物标志物。©2017 by John Wiley &儿子,Inc。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

CyTOF Measurement of Immunocompetence Across Major Immune Cell Types

CyTOF Measurement of Immunocompetence Across Major Immune Cell Types

CyTOF Measurement of Immunocompetence Across Major Immune Cell Types

The central role of the immune system is becoming appreciated in a wide variety of diseases. Cancer immunotherapy is one area that has yielded much recent success, although not all patients benefit equally. At the same time, recent studies have highlighted the heterogeneity of the human immune system. Despite this heterogeneity, we do not routinely measure immune competence in clinical practice, and there are no consensus assays of healthy immune function. Using mass cytometry (CyTOF), we can simultaneously detect ∼40 markers to identify various cell subsets and determine their function by the expression of cytokines, cytotoxicity, and activation markers. This can help assess ‘immunocompetence’ and facilitate better implementation of immunotherapies, both in specific disease settings and perhaps eventually as a prognostic tool in healthy subjects. Here we introduce the concepts behind this assay and provide a protocol that we have successfully implemented to identify possible predictive biomarkers of immunotherapy outcome. © 2017 by John Wiley & Sons, Inc.

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来源期刊
Current Protocols in Cytometry
Current Protocols in Cytometry Health Professions-Medical Laboratory Technology
自引率
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期刊介绍: Published in affiliation with the International Society for Advancement of Cytometry, Current Protocols in Cytometry is a "best practices" collection that distills and organizes the absolute latest techniques from the top cytometry labs and specialists worldwide. It is the most complete set of peer-reviewed protocols for flow and image cytometry available.
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