利用RNA指纹技术探测RNA折叠途径

Q4 Chemistry
Sarah A. Woodson
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引用次数: 0

摘要

该单元提供了使用天然聚丙烯酰胺凝胶电泳来区分RNA折叠和展开构象的协议。它对于研究可以在几分钟或几秒钟内交换的构象是有用的,因此难以通过基于解的方法进行研究。已分离并固定在凝胶基质中的构象可以用于研究催化活性,无论是否从凝胶中洗脱。该方法可应用于多种催化rna和rna -蛋白复合物。©2017 by John Wiley &儿子,Inc。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Probing RNA Folding Pathways by RNA Fingerprinting

This unit provides protocols for using native polyacrylamide gel electrophoresis to distinguish folding and unfolding conformers of RNA. It is useful for studying conformers that can exchange in a period of minutes or seconds, and that are thus difficult to study by solution-based methods. Conformers that have been separated and immobilized in the gel matrix can be used to study catalytic activity with or without being eluted from the gel. The method can be applied to a wide variety of catalytic RNAs and RNA-protein complexes. © 2017 by John Wiley & Sons, Inc.

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来源期刊
Current Protocols in Nucleic Acid Chemistry
Current Protocols in Nucleic Acid Chemistry Chemistry-Organic Chemistry
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期刊介绍: Published in association with International Society for Nucleosides, Nucleotides & Nucleic Acids (IS3NA) , Current Protocols in Nucleic Acid Chemistry is equally valuable for biotech, pharmaceutical, and academic labs. It is the resource for designing and running successful research projects in the rapidly growing and changing field of nucleic acid, nucleotide, and nucleoside research.
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