Jecko Thachil, Giuseppe Lippi, Emmanuel J Favaloro
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引用次数: 50
摘要
d -二聚体已成为常用的凝血检查之一，特别是在静脉血栓栓塞排除设置。适当的d -二聚体检测显著减少了这种临床情况下的放射学检查次数。d -二聚体检测在全身性凝血障碍的诊断过程中也起着重要的作用，特别是弥散性血管内凝血与其他凝血检查相结合。然而，在没有很好地理解与测试相关的技术问题的情况下，广泛的d -二聚体测试最近引起了一些关注。本文综述了d -二聚体形成的基本生理学，常规评估的不同方法，临床医生和实验室科学家目前面临的问题，以及商业免疫测定法统一的重要需求。
D-Dimer Testing: Laboratory Aspects and Current Issues.
D-dimer has become one of the commonly requested coagulation tests, especially in the venous thromboembolism rule out setting. Appropriate D-dimer testing has significantly decreased the number of radiological investigations in this clinical scenario. D-dimer testing also plays an important role in the diagnostic process of the systemic coagulation disorders, especially disseminated intravascular coagulation in conjunction with other coagulation tests. However, widespread D-dimer testing without good understanding of the technical issues related to the test has recently caused some concerns. This review aims to address the basic physiology of D-dimer formation, the different methods available for its routine assessment, the current problems that both clinicians and laboratory scientists face with this test, and the important need for harmonization of commercial immunoassays.
期刊介绍：
For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
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