从皮肤活检中生成无xeno、符合cgmp的患者特异性iPSCs

Q2 Biochemistry, Genetics and Molecular Biology

Current Protocols in Stem Cell Biology Pub Date : 2017-08-14 DOI:10.1002/cpsc.30

Luke A. Wiley, Kristin R. Anfinson, Cathryn M. Cranston, Emily E. Kaalberg, Malia M. Collins, Robert F. Mullins, Edwin M. Stone, Budd A. Tucker

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引用次数: 16

摘要

本单元描述了从遗传性视网膜退行性失明患者中产生临床级患者特异性诱导多能干细胞(iPSC)衍生视网膜细胞的方案。具体来说，我们描述了如何在ISO 5级环境中使用无xeno试剂，分离和培养真皮成纤维细胞，生成iPSCs，并通过3-D分化获得自体视网膜细胞。本文描述的用于分离真皮成纤维细胞和生成iPSCs的通用方法可用于任何疾病、组织或感兴趣的细胞类型。©2017 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Generation of Xeno-Free, cGMP-Compliant Patient-Specific iPSCs from Skin Biopsy

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Generation of Xeno-Free, cGMP-Compliant Patient-Specific iPSCs from Skin Biopsy

This unit describes protocols for the generation of clinical-grade patient-specific induced pluripotent stem cell (iPSC)–derived retinal cells from patients with inherited retinal degenerative blindness. Specifically, we describe how, using xeno-free reagents in an ISO class 5 environment, one can isolate and culture dermal fibroblasts, generate iPSCs, and derive autologous retinal cells via 3-D differentiation. The universal methods described herein for the isolation of dermal fibroblasts and generation of iPSCs can be employed regardless of disease, tissue, or cell type of interest. © 2017 by John Wiley & Sons, Inc.

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来源期刊

Current Protocols in Stem Cell Biology Biochemistry, Genetics and Molecular Biology-Cell Biology

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发文量

期刊介绍： Published in affiliation with the International Society for Stem Cell Research (ISSCR), Current Protocols in Stem Cell Biology (CPSC) covers the most fundamental protocols and methods in the rapidly growing field of stem cell biology. Updated monthly, CPSC will constantly evolve with thelatest developments and breakthroughs in the field. Drawing on the expertise of leading researchers from around the world, Current Protocols in Stem Cell Biology includes methods and insights that will enhance the progress of global research.