如何加快聚合酶链反应

Q1 Biochemistry, Genetics and Molecular Biology

Biomolecular Detection and Quantification Pub Date : 2017-06-01 DOI:10.1016/j.bdq.2017.05.002

Stephen A. Bustin

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引用次数: 31

摘要

减少在今天的qPCR循环器上运行qPCR分析所花费的时间是相当简单的，不需要专门的试剂或仪器。作为新系列简短技术报告的第一篇文章，我证明了有可能显著降低变性温度和循环时间，同时保留原始qPCR条件的敏感性和特异性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

How to speed up the polymerase chain reaction

查看原文本刊更多论文

How to speed up the polymerase chain reaction

Reducing the time taken to run qPCR assays on today’s qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions.

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来源期刊

Biomolecular Detection and Quantification Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

14.20

自引率

0.00%

发文量

审稿时长

8 weeks