从冷冻保存的沃顿果冻中提取间充质干细胞方法的比较研究。

IF 1.1 Q4 CELL & TISSUE ENGINEERING

Journal of Stem Cells & Regenerative Medicine Pub Date : 2017-05-30 eCollection Date: 2017-01-01

Peng Yew Kenny Boey, Say Liang Daniel Lim, Kin Fai Tang, Ming Ming Li, Andrew Krishna Ekaputra, Prosanto Kumar Chowdhury, Rajat Anand Gopal Mukherjee, Jennifer Teo, Arvin C Faundo, Yoke Fong Chiew

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引用次数: 0

摘要

沃顿果冻（WJ）是一种成熟的间充质干细胞（MSC）来源。我们比较了从冷冻保存的组织中提取WJ-间充质干细胞的3种方法，并确定与外植体法和机械消化法相比，酶解WJ可获得存活率最高的间充质干细胞。经酶解释放的WJ-间充质干细胞符合国际细胞治疗学会（ISCT）的标准：显示塑性粘附，共表达CD73、CD90、CD105，造血系细胞标记阴性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Comparative study of the methods of extracting mesenchymal stem cells from cryopreserved Wharton's Jelly.

本刊更多论文

Comparative study of the methods of extracting mesenchymal stem cells from cryopreserved Wharton's Jelly.

The Wharton's Jelly (WJ) is an established source of mesenchymal stem cells (MSC). We compared 3 methods of extracting WJ-MSC from cryopreserved tissue and determined that enzymatic digestion of the WJ yielded the most viable MSC, compared to the explant and mechanical digestion methods. The enzymatically-released WJ-MSC conformed to the International Society for Cellular Therapy (ISCT) criteria: displayed plastic-adherence, co-expressed CD73, CD90, CD105 and were negative for hematopoietic lineage cell markers.

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来源期刊

Journal of Stem Cells & Regenerative Medicine CELL & TISSUE ENGINEERING-

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

14 weeks