Xiao-Fei Guo, Wen-Ping Hu, Xian-Zheng Lang, Qiu-Ling Li, Xiang-Yu Wang, Ran Di, Qiu-Yue Liu, Xiao-Lin Liu, Yong-Fu An, Ming-Xing Chu
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AA (0.3633), AB (0.4644) and BB (0.1723) genotypes were detected at G5503A site, CC (0.3483), CD (0.4906) and DD (0.1611) genotypes were found at G5746C in Chinese Holstein cows. Least squares mean of SCS for individuals with BB genotype was significantly lower than that with AA and AB genotype (<i>p</i> < 0.01), and that with AB genotype was significantly lower than that with AA (<i>p</i> < 0.05). There was no significant difference among individuals with CC, CD and DD genotypes (<i>p</i> > 0.05). 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引用次数: 0
摘要
背景：α-1-抗胰蛋白酶（α1-AT）可能会影响奶牛对乳腺炎的易感性，因为它可能在保护乳铁蛋白免受乳腺蛋白分解方面发挥作用。牛奶体细胞评分（SCS）是牛奶体细胞数的对数变换，被广泛用作乳腺炎的评估指标。为研究中国荷斯坦奶牛α1-AT基因与SCS的关系，采用PCR-SSCP、DNA测序、PCR-RFLP和CRS-PCR技术检测α1-AT基因的单核苷酸多态性位点：结果：在α1-AT基因的G5503A和G5746C上发现了两个多态性位点。在中国荷斯坦奶牛的 G5503A 位点上检测到 AA（0.3633）、AB（0.4644）和 BB（0.1723）基因型，在 G5746C 位点上检测到 CC（0.3483）、CD（0.4906）和 DD（0.1611）基因型。BB 基因型个体的 SCS 最小二乘法平均值明显低于 AA 和 AB 基因型个体（p p p > 0.05）。BBDD基因型组合个体的SCS最小二乘法平均值明显低于AACC和AACD基因型组合个体（p 结论：BBDD基因型组合个体的SCS最小二乘法平均值明显低于AACC和AACD基因型组合个体（p）：统计分析表明，α1-AT 的 B 等位基因和 BBDD 基因型组合可提高奶牛的乳腺炎抵抗力。
Two single nucleotide polymorphisms sites in α1-AT gene and their association with somatic cell score in Chinese Holstein cows.
Background: Alpha 1-antitrypsin (α1-AT) may affect the susceptibility of mastitis in dairy cattle for its possible role in the protection of lactoferrin from proteolytic degradation in the mammary. Milk somatic cell score (SCS) is a logarithmic transformation of the milk somatic cell count widely used as an index to evaluate mastitis. To study the relationships of α1-AT gene and SCS in Chinese Holstein cows, methods of PCR-SSCP, DNA sequencing, PCR-RFLP, and CRS-PCR technologies were used to detect single nucleotide polymorphisms sites in α1-AT gene.
Results: Two polymorphic sites at G5503A and G5746C of α1-AT gene were found. AA (0.3633), AB (0.4644) and BB (0.1723) genotypes were detected at G5503A site, CC (0.3483), CD (0.4906) and DD (0.1611) genotypes were found at G5746C in Chinese Holstein cows. Least squares mean of SCS for individuals with BB genotype was significantly lower than that with AA and AB genotype (p < 0.01), and that with AB genotype was significantly lower than that with AA (p < 0.05). There was no significant difference among individuals with CC, CD and DD genotypes (p > 0.05). Least squares mean of SCS for individuals with BBDD genotype combination were significantly lower than those with AACC and AACD (p < 0.05).
Conclusions: Statistical analysis indicated that B allele and BBDD genotype combination of α1-AT can improve mastitis resistance in dairy cattle.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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