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引用次数: 11
摘要
硫酸肝素6- o -磺化酶sulf1和sulf2调节多种细胞过程和器官发育。硫调节一系列肝素硫酸盐依赖的细胞外通路,包括成纤维细胞生长因子、骨形态发生蛋白和无翼/wnt信号通路。已知的硫转录表达模式和功能实验表明,硫与哺乳动物的软骨形成和肌肉再生有关。本文描述了非洲爪蟾(Xenopus laevis)在发育的前肢和后肢中硫化物和硫化物的表达模式,并在再生后肢中发现了硫化物转录物的新表达,其中硫化物在增殖囊胚中有显著表达,而硫化物在再发育的顶表皮脊中有短暂表达。这些发现进一步表明硫酸盐参与了两栖动物成功的肢体再生。
Distinct patterns of endosulfatase gene expression during Xenopus laevis limb development and regeneration.
The heparan sulfate 6-O-endosulfatases sulf1 and sulf2 regulate multiple cellular processes and organ development. Sulfs modulate a range of heparan-sulfate-dependent extracellular pathways, including the fibroblast growth factor, bone morphogenetic protein, and wingless/wnt signaling pathways. Known patterns of sulf transcript expression together with functional experiments have implicated the sulfs in chondrogenesis and muscle regeneration in mammals. Here, we describe the expression patterns of Xenopus laevis sulf1 and sulf2 in developing forelimbs and hindlimbs and demonstrate novel expression of the sulf transcripts in the regenerating hindlimbs, with prominent sulf2 expression in the proliferating blastema and transient expression of sulf1 in the redeveloping apical epidermal ridge. These findings further suggest involvement of the sulfs in successful limb regeneration in amphibians.